Salvianolic acid D
Salvianolic acid and notoginseng triterpenes can promote EA-hy926 and expression of protein, have protective effects on angiogenesis in EA-hy926 cells in vitro.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C)
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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A Platelet/CMC coupled with offline UPLC-QTOF-MS/MS for screening antiplatelet activity components from aqueous extract of Danshen[Pubmed: 26355772
Platelets play crucial roles in thromboembolic and cardiovascular disease. The main platelets membrane receptors include adenosine diphosphate receptors, thrombin receptors, thromboxane prostanoid receptors and collagen receptors. In this study, a Platelet/CMC coupled with offline UPLC-QTOF-MS/MS system was built to screen antiplatelet activity components from aqueous extract of Danshen, which serve as an agent of antiplatelet aggregation in Traditional Chinese Medicine. Rosmarinic acid, lithospermic acid, salvianolic acid B, two isomers of salvianolic acid B, salvianolic acid C, Salvianolic acid D and salvianolic acid H/I were identified as the potential antiplatelet activity components. Moreover, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid C and danshensu were tested in platelet aggregation in vitro assay. The results suggested their retention time was closely related to the antiplatelet aggregation activities. This study provides a rapid, effective and novel method for screening the potential antiplatelet activity components from Chinese herb medicines.
Keywords: Cell membrane chromatography; Danshen; Platelet; UPLC-QTOF-MS/MS.
Acta Pharmaceutica Sinica BVolume 5, Issue 3, May 2015, Pages 246–253
Pharmacokinetic study of salvianolic acid D after oral and intravenous administration in rats[Reference: WebLink
METHODS AND RESULTS:
A sensitive, specific and rapid LC-MS method was developed and validated for the determination of Salvianolic acid D (SalD) in rat plasma. This method used a single quadrupole mass spectrometer with an electrospray ionization (ESI) source. A single ion monitoring scanning (SIM) mode was employed. It showed good linearity over the concentration range from 3.3 to 666.7 ng/mL for the determination of Salvianolic acid D. The R.S.D.% of intra-day and inter-day precision values were no more than 7.69%, and the accuracy was within 91%−104% at all quality control levels.
This LC-MS method was applied to the pharmacokinetic study of Salvianolic acid D in rats. A two-compartmental model analysis was employed. The bioavailability of Salvianolic acid D was calculated to be 4.159%±0.517%.
《China Pharmacy》 2012-08
Isolation and Identification of Salvianolic Acid D and Simultaneous Determination of 4 Salvianolic Acids in Danshen Infusion[Reference: WebLink
To establish the method for the isolation and identification of Salvianolic acid D and simultaneous determination of tanshinol,protocatechuic aldehyde,Salvianolic acid D and B in Danshen infusion.
METHODS AND RESULTS:
RP-HPLC method was adopted for isolation and content determination.The determination was performed on Kromasil C18 column with mobile phase consisted of acetonitrile-1% glacial acetic acid at the flow rate of 1.0 mL·min-1.The injection volume was 10 μL and column temperature was 30 ℃.The detection wavelength was set at 281 nm. Salvianolic acid D had been succeddfully isolated and indentified.The linearities of tanshinol,protocatechuic aldehyde,Salvianolic acid D,salvianolic acid B were 60.9～975.0,8.7～139.0,11.0～175.8,2.7～43.2 μg·mL-1.The average recoveries rate were 99.1%,100.2%,98.3%,100.4%,102.5%,101.1%,101.4%,97.0%,102.1%,100.6%,99.2%,101.3%,and the RSDs were 0.94%,0.97%,2.78%,1.07%.
The method is simple,rapid and specific.It may be suitable for the quality control of Danshen infusion.