Oroxylin A 7-O-beta-D-glucuronide methyl ester
Oroxylin A 7-O-beta-D-glucuronide methyl ester possibly possesses the potential immune activities.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chinese Traditional and Herbal Drugs,2006.
Study on flavonoids from Nicandra physaloides and their immune activities.[Reference:
WebLink]
To investigate the flavonoids from the fruits of Nicandra physaloides and clarify the immune activities of the isolated compounds.
METHODS AND RESULTS:
The separations and purifications were taken by silica gel and ODS chromatogram columns as well as preparative HPLC, and the structural identification based on physicochemical property, ¹H-NMR, and ¹³C-NMR as well as HR-MS data. And the immune activities were evaluated by mice splenic lymphocyte proliferation model induced by LPS. Thirteen compounds were obtained from the butanol fraction of the 70% ethanol extract of N. physaloides fruits, which were identified as 3''-hydroxy-puerarin (1), puerarin-6″-O-glucoside (2), daidzein 8-C-[α-D-apiofranosyl-(1→6)]-β-D-glucopyranoside (3), puerarin (4), 3''-methoxyl-puerarin (5), chrysin 6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (6), Oroxylin A 7-O-beta-D-glucuronide methyl ester (7), daidzin (8), baicalin (9), quercetin-3-O-β-D-galactopyranoside (10), quercetin 3-rutinoside (11), kaempferol 3-rutinoside (12), and quercetin 3-glucoside (13), respectively. The results indicated that compounds 1-3, 7, 9-10, and 12-13 could inhibit the proliferation of mice spleen lymphocytes and 6 could promote the proliferation in the influence of mice spleen lymphocytes proliferation induced by LPS.
CONCLUSIONS:
Compounds 1-7 are isolated from the plants of Solanaceae for the first time, compounds 8-13 are obtained from the plants of Nicandra Adans. firstly. And the compounds 1-3, 6, 7, 9-10, and 12-13 possibly possess the potential immune activities.
