Celosin H, celosin I, celosin J could be used as chemical markers for the quality control of C. argentea seeds.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C)
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Dereplication-guided isolation of novel hepatoprotective triterpenoid saponins from Celosiae Semen by high-performance liquid chromatography coupled with electrospray ionization tandem quadrupole-time-of-flight mass spectrometry.[Pubmed: 27721071
Although natural products (NPs) from ethnomedical plants have played a vital role in modern drug discovery, separation and purification of bioactive compounds from plant extract is still challenging.
METHODS AND RESULTS:
In this study, a dereplication strategy using HPLC-QTOF-MS was employed to rapidly discover and highly targeted isolate the novel hepatoprotective triterpenoid saponins from the methanol extract of Celosiae Semen. Firstly, four known saponins, i.e. celosin H, celosin I, Celosin J, and pseudoginsenoside RT1 were selected as model compounds, and their fragmentation patterns in ESI-QTOF-MS/MS were characterized. Secondly, an HPLC-QTOF-MS/MS method was applied to chemically screen the saponins of interest, and thereby to guide the subsequent fraction and isolation procedure. Thirdly, the targeted isolation of desired compounds afforded two new triterpenoid saponins namely celosin K (1) and celosin L (2), which were structurally elucidated by combination of extensive NMR spectroscopic and chemical analyses. Finally, the protective effects of compounds 1 and 2 against APAP-induced hepatotoxicity in HepG2 cells were evaluated.
These results indicate that the HPLC-QTOF-MS-guided isolation is an efficient methodology for isolating new NPs from medicinal plants through improving selectivity in separation and purification process.
J Asian Nat Prod Res. 2014;16(3):240-7.
New oleanane-type triterpenoid saponins isolated from the seeds of Celosia argentea.[Pubmed: 24456247
METHODS AND RESULTS:
Three new oleanane-type triterpenoid saponins named celosins H, I, and J were isolated from the seeds of Celosia argentea L. Their structures were characterized as 3-O-Î²-D-xylopyranosyl-(1 â†’ 3)-Î²-D-glucuronopyranosyl-polygalagenin 28-O-Î²-D-glucopyranosyl ester, 3-O-Î²-D-glucuronopyranosyl-medicagenic acid 28-O-Î²-D-xylcopyranosyl-(1 â†’ 4)-Î±-L-rhamnopyranosyl-(1 â†’ 2)-Î²-D-fucopyranosyl ester, and 3-O-Î²-D-glucuronopyranosyl-medicagenic acid 28-O-Î±-L-arabinopyranosyl-(1 â†’ 3)-[Î²-D-xylcopyranosyl-(1 â†’ 4)]-Î±-L-rhamnopyranosyl-(1 â†’ 2)-Î²-D-fucopyranosyl ester by NMR, MS, and chemical evidences, respectively.
In our opinion, celosin H, celosin I, Celosin J could be used as chemical markers for the quality control of C. argentea seeds.