Hannokinol

Planta Med. 2008 Jun;74(8):867-9.

Inhibitory constituents of lipopolysaccharide-induced nitric oxide production in BV2 microglia isolated from Amomum tsao-ko.[Pubmed: 18523923 ]

A methanolic extract of the fruits of AMOMUM TSAO-KO (Zingiberaceae) significantly attenuated nitric oxide production in lipopolysaccharide-simulated BV2 microglia.
METHODS AND RESULTS:
Two new bicyclic nonanes characterized as 6,7-dihydroxy-indan-4-carbaldehyde ( 1) and 6-hydroxy-indan-4-carbaldehyde ( 2) were isolated with the eleven known compounds 6,7-dihydroxy-3,7-dimethyloct-2-enoic acid ( 3), tsaokoin ( 4), isotsaokoin ( 5), 8-oxogeraniol ( 6), P-menth-1-ene-5,6-diol ( 7), 3alpha-hydroxycarvotagenone ( 8), tsaokoarylone ( 9), 1,7-bis(4-hydroxy-3-methoxyphenyl)-4,6-heptadien-3one ( 10), (+)-Hannokinol ( 11), MESO-Hannokinol ( 12) and hannokinin ( 13), from the fruits of A. TSAO-KO using bioactivity-guided fractionation.
CONCLUSIONS:
All thirteen compounds significantly inhibited lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 1 microM to 100 microM.

Chinese Journal of Experimental Traditional Medical Formulae, 2015 , 21 (3) :37-40.

Identification of Chemical Constituents Occurring in Leaves of Alpinia officinarum[Reference: WebLink]

The current study aimed to identify the chemical constituents occurring in the leaves of Alpinia officinarum.
METHODS AND RESULTS:
The collected leaves of A. officinarum were dried in an electric thermostatic drying oven at 40° C overnight. The freshly dried leaves were smashed using a universal high-speed smashing machine and then sieved manually by an 80 mesh. The resulting fine powders and residue were mixed evenly. Twelve flavonoids were chrysin,pinocembrin,tectochrysin,apigenin,galangin,3-methylgalangin,acacetin,kaempferol,kaempferide,quercetin,isorhamnetin and rutin,respectively. Four diarylheptanoids included yakuchinone A,oxyphyllacinol,hexahydrocurcumin and Hannokinol.
CONCLUSIONS:
Sixteen chemicals were identified using LC-MS / MS and some unknown diarylheptanoids need to be identified in the future.