Euphorbia factor L2
Euphorbia factor L2 shows cytotoxic activity against lung carcinoma A549 cells, it induces apoptosis through a mitochondrial pathway.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Acta Pharm Sin B. 2017 Jan;7(1):59-64.
Euphorbia factor L2 induces apoptosis in A549 cells through the mitochondrial pathway.[Pubmed:
28119809 ]
Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed (the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Euphorbia factor L2 against lung carcinoma A549 cells and the mechanism by which apoptosis is induced.
METHODS AND RESULTS:
We analyzed the cytotoxicity and related mechanism of Euphorbia factor L2 with an MTT assay, an annexin V-FITC/PI test, a colorimetric assay, and immunoblotting. Euphorbia factor L2 showed potent cytotoxicity to A549 cells. Euphorbia factor L2 led to an increase in reactive oxygen species (ROS) generation, a loss of mitochondrial electrochemical potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase, suggesting that Euphorbia factor L2 induced apoptosis through a mitochondrial pathway.
CONCLUSIONS:
The cytotoxic activity of Euphorbia factor L2 in A549 cells and the related mechanisms of apoptotic induction provide support for the further investigation of caper euphorbia seeds.
Journal of Pharmaceutical and Biomedical Analysis.2013 Jan 18;72(18):299–305.
A sensitive liquid chromatography–mass spectrometry method for simultaneous determination of three diterpenoid esters from Euphorbia lathyris L. in rat plasma.[Reference:
WebLink]
A selective and sensitive liquid chromatography–mass spectrometry (LC–MS) method was developed for the simultaneous determination of Euphorbia factor L1, Euphorbia factor L2 and Euphorbia factor L3 from Euphorbiae semen in rat plasma.
METHODS AND RESULTS:
Larotaxel was added to a 200 μL plasma sample as the internal standard (IS). The plasma sample was extracted by 2 mL ether and separated on an Elite C18 column (150 mm × 4.6 mm, 5 μm) with the mobile phase of methanol–water performing gradient elution within 11.0 min. All the three analytes were detected in the selected ion monitoring (SIM) mode with positive electrospray ionization. The calibration curves were linear in the range of 2.0–200 ng/mL and the lower limits of quantification (LLOQ) were 2.0 ng/mL for all the three analytes. The intra-day and inter-day precisions were all within 8.6% and 14.6% for the three analytes, while the accuracy was less than 9.7% and 7.5%, respectively.
CONCLUSIONS:
The validated method was firstly and successfully applied to the pharmacokinetic study of Euphorbia factor L1, Euphorbia factor L2 and Euphorbia factor L3 from Euphorbiae semen after oral administration in rat plasma.
