Demethylwedelolactone

Phytother Res. 2003 Apr;17(4):420-1.

Trypsin inhibitory effect of wedelolactone and demethylwedelolactone.[Pubmed: 12722155]

Wedelolactone (WL) and Demethylwedelolactone (DWL) isolated from Eclipta alba were tested in the trypsin inhibition bioassay (in vitro).
METHODS AND RESULTS:
Both compounds showed potent activity. IC(50) values of WL and DWL were found to be 2.9 and 3.0 microg/mL respectively.

Eur J Med Chem. 2012 Oct;56:361-7.

Demethylwedelolactone derivatives inhibit invasive growth in vitro and lung metastasis of MDA-MB-231 breast cancer cells in nude mice.[Pubmed: 22926226]

The anticancer properties of Demethylwedelolactone (DWEL) and wedelolactone (WEL), which are naturally occurring coumestans, have not been well characterized. In this study, we investigated the anti-invasive effects of synthetic WEL and Demethylwedelolactone on human MDA-MB-231 breast cancer cells.
METHODS AND RESULTS:
We found that WEL and Demethylwedelolactone inhibited the anchorage-independent growth and also suppressed cell motility and cell invasion of MDA-MB-231 cells. In addition, WEL and Demethylwedelolactone reduced the activity and expression of matrix metalloproteinases (MMPs) involved in blocking the IκB-α/NFκB and MEK/ERK signaling pathways in MDA-MB-231 cells. Furthermore, Demethylwedelolactone suppressed the metastasis and lung colonization of the tumor cells in the nude mice.
CONCLUSIONS:
Altogether, these data suggest that Demethylwedelolactone derivatives exert anti-invasive growth effect on breast cancer cells.