4-O-Methylbutein | CAS:13323-67-6 | Manufacturer ChemFaces

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

J Ethnopharmacol.2018, 210:88-94

Journal of Phytopathology2021, 169,Issue11-12.

Anticancer Res.2020, 40(10):5529-5538.

Int J Mol Sci.2017, 18(12)

Applied Biological Chemistry2022, 65(12)

Front Immunol.2018, 9:2091

Phytochemistry.2021, 181:112539.

Anal Biochem.2019, 569:10-15

Molecules.2022, 27(7):2093.

Metabolites.2020, 11(1):E11.

Free Radic Biol Med. 2015 Jan;78:135-46.

Identification of heme oxygenase-1 stimulators by a convenient ELISA-based bilirubin quantification assay.[Pubmed: 25462643 ]

The upregulation of heme oxygenase-1 (HO-1) has proven to be a useful tool for fighting inflammation. In order to identify new HO-1 inducers, an efficient screening method was developed which can provide new lead structures for drug research.
METHODS AND RESULTS:
We designed a simple ELISA-based HO-1 enzyme activity assay, which allows for the screening of 12 compounds in parallel in the setting of a 96-well plate. The activity of known HO-1 inducers was confirmed, while the chalcones cardamonin, flavokawain A, calythropsin, 2',3,4'-trihydroxy-4-methoxychalcone (4-O-Methylbutein,THMC), and 2',4'-dihydroxy-3,4-dimethoxychalcone (DHDMC) were identified as new potent HO-1 inducers. The highest inductive power after 6h incubation was found at 10 uM for DHDMC (6.1-fold), carnosol (3.9-fold), butein (3.1-fold), THMC (2.9-fold), and zerumbone (2.5-fold). Moreover, the time dependence of HO-1 protein production for DHDMC was compared to its enzyme activity, which was further evaluated in the presence of lipopolysaccharide and the specific HO-1 inhibitor tin protoporphyrin IX.
CONCLUSIONS:
Taken together, we developed a convenient and highly sensitive ELISA-based HO-1 enzyme activity assay, allowing the identification and characterization of molecules potentially useful for the treatment of inflammatory and autoimmune diseases.

Phytochemistry,1975,14(7):1621-3.

Flavonoids and polyacetylenes in Dahlia tenuicaulis[Reference: WebLink]

METHODS AND RESULTS:
5,7,4′-Trimethoxyflavanone,5-hydroxy-7,4′-dimethoxyflavanone,2′-hydroxy-4,4′,6′-trimeth-oxychalcone, and a new naturally occurring compound, 5,7,4′-trimethoxyflavan-4-ol have been isolated from the leaves of Dahlia tenuicaulis Sorensen. Two chalcones, 4,2′,4′-trihydroxychalcone and 3,2′,4′-trihydroxy-4-methoxychalcone(4-O-Methylbutein), and 5-hydroxy-7,4′-dimethoxyflavanone have been isolated from the flower heads.
CONCLUSIONS:
Minute amounts of the polyacetylene 1,3-diacetoxy-tetradeca-4,6-diene-8,10,12-triyne have been found in both leaves and flower heads, whereas 1-acetoxy-tetradeca-4,6-diene-8,10,12-triyne was present only in the flower heads.

Product Name	4-O-Methylbutein
CAS No.:	13323-67-6
Catalog No.:	CFN96387
Molecular Formula:	C₁₆H₁₄O₅
Molecular Weight:	286.3 g/mol
Purity:	>=98%
Type of Compound:	Flavonoids
Physical Desc.:	Yellow powder
Targets:	HO-1
Source:	The flower heads of Dahlia tenuicaulis Sorensen.
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
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