Pyrocincholic acid methyl ester
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Helvetica Chimica Acta, 2009, 92(4):638-644.
Triterpenoids and Saponins from the Leaves of Uncaria hirsuta.[Reference:
WebLink]
METHODS AND RESULTS:
Three new triterpenoids, (3β)-3-hydroxy-27-noroleano-13(28)-lactone (1), (22α)-22-hydroxy-3-oxours-12-ene-27,28-dioic acid (4), and (3β)-3-(β-D-glucopyranosyloxy)-12-oxopyroquinovic acid β-D-glucopyranosyl ester (7), together with four known compounds, pyrocincholic acid ethyl ester (2), pyrocincholic acid (3), quinovic acid (5), and (3β)-3-(β-D-quinovopyranosyloxy)pyrocincholic acid β-D-glucopyranosyl ester (6), were isolated from the EtOH extract of the leaves of Uncaria hirsuta Haviland. Their structures were determined by means of spectroscopic analyses, including HR-ESI-MS and 2D-NMR techniques.
CONCLUSIONS:
The configuration of 1 was confirmed by X-ray analysis; 2 is an artefact, as verified by the HPLC and LC/MS analysis.
