N1,N10-Bis(p-coumaroyl)spermidine
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Phytochemistry, 1998, 47(3):375-387.
The biosynthesis of lunarine in seeds of Lunaria annua.[Reference:
WebLink]
METHODS AND RESULTS:
Using an optimized precursor feeding system, the biosynthesis of the alkaloid lunarine in Lunaria annua seeds was investigated. The synthesis of radioactively labelled precursors for the application experiments is described. Lunarine was shown to be synthesized by stereo-selective phenol-oxidative coupling of N1,N10-Bis(p-coumaroyl)spermidine. p-Coumaric acid for the biosynthesis of this dimer is formed from L-phenylalanine via trans-cinnamic acid. The polyamine moiety of lunarine, spermidine, and its immediate precursor putrescine are preferentially synthesized from arginine.
CONCLUSIONS:
Enzyme studies with microsomes of Lunaria annua seeds indicated that a cytochrome P-450 enzyme might be responsible for the phenol-oxidative coupling of N1,N10-bis (p-coumaroyl)spermidine yielding the hexahydrodibenzofuran ring of lunarine.
spermidine-CFN99248.jpg "N1,N10-Bis(p-coumaroyl)spermidine")
