Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Chemical Research in Chinese Universities,2003,24(3):436-41.
Chemical Constituents from the Roots of Biondia Hemsleyana[Reference:
WebLink]
Nineteen I compounds were isolated from the,ethanol extract of the roots of Biondia hemsleyana (Warb.) Tsiang through. the repeated column chromatography on normal and reversed phase silica gel.
METHODS AND RESULTS:
By the spectroscopic and chemical evidence, their structures were elucidated as alpha-amyrin-3beta-acetate (1), Lupeol 3-hydroxyoctadecanoate (2), lupeol 3, 5-dihydroxyeicosanoate (3), cycloeucalenol (4), 4-methoxy salicylic aldehyde(5), 4-methoxy salicylic acid(6), isovanillin(7), scopoletin(8), isovanillic acid(9), cleomiscosin A (10), 2-hydroxmethyl-5-methoxylphenyl-O-beta-D-glucopyranoside (11), pregn-4-ene-3, 20-dione (12), neridienone (13), daucosterol(14), pregn-5-ene-3beta, 17alpha, 20alpha-triol-20-O-beta-D-canaropyranoside (15), periplogenin-3-O-beta-D-gluco pyranosyl-(1-->4)-beta-D-cymaropyranoside (16), pregn-5-ene-3beta, 20 (S)-diol-3-O-[2-O-acetyl-beta-D-digitalopyranosyl (1-->4)-beta-D-cymaropyranosyl]-20-O-beta-D-glucopyranosyl-(1-->6)-beta- D-glucopyranosyl-(1-->4)-beta-D-digitalo pyranoside(17), pregn-5-ene-3beta, 20(S)-diol-20-O-alpha-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside(18 , biondianoside C) and pregn-5-ene-3beta, 20(S)-diol-3-O-[beta-D-glucopyranosyl]-20-O-beta-D-glucopyranosyl-(1-->6 )-beta-D-glucopyranoside (19, biondianoside D) respectively.
CONCLUSIONS:
All compounds were isolated from this genus for the first time and 3, 11, 18 and 19 were new compounds among them.
