Isovitexin 2''-O-arabinoside
Isovitexin 2''-O-arabinoside is a natural product from Secale cereale.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Journal of Plant Physiology, 1988, 133(2):178-182.
Biochemical and Immunological Characterization of Chalcone Synthase from Rye Leaves.[Reference:
WebLink]
As part of investigations on the role and the location of the key enzyme of flavonoid biosynthesis, chalcone synthase (CHS) in leaf tissues of Gramineae, CHS from rye (Secale cereale L.) was purified and characterized biochemically and immunologically.
METHODS AND RESULTS:
Km-values for the substrates p-coumaroyl-CoA and caffeoyl-CoA were 0.6 and 1.45 μM, respectively, the corresponding Km-values for malonyl-CoA were 1.4 and 1.5 μM. The pH-optimum for the formation of 2′,4,4′,6′-tetrahydroxychalcone was 8 and for the formation of 2′,3,4,4′,6′-pentahydroxychalcone, 6.5. A 50% reduction in enzyme activity was caused by 9 μM apigenin, 13 μM luteolin, 36 μM CoA, 45 μM naringenin, 45 μM eriodictyol, and 62 μM Isovitexin 2''-O-arabinoside. SDS-PAGE analysis of the purified enzyme revealed two peptides with apparent molecular weights of 43 and 44 kd, respectively.
CONCLUSIONS:
On western blots run with the purified enzyme as well as with crude enzyme extracts, both peptides were immunostained by polyclonal antibodies raised against rye or parsley CHS. The recognition of these peptides by single monoclonal antibodies demonstrated that both peptides were similar and derived from CHS. Applied to western blots run with CHS-containing extracts of parsley, spinach, pea, maize, and oat, the same antibodies labelled only a single peptide, or in some cases probably even more than two peptides.
Phytochemistry, 1983, 22(11):2627-2628.
Two isovitexin 2″-O-glycosides from primary leaves of Secale cereale.[Reference:
WebLink]
Two isovitexin 2″-O-glycosides have been isolated from primary leaves of rye and identified as Isovitexin 2''-O-arabinoside and isovitexin 2.
