Vicenin -1

Journal of the Korean Society for Applied Biological Chemistry, 2015, 58(3):365-371.

Plant-derived molecules from Saussurea grandifolia as inhibitors of aldose reductase.[Reference: WebLink]

Saussurea grandifolia was tested for aldose reductase (AR) inhibition as part of a search for potentially therapeutic natural compounds.
METHODS AND RESULTS:
Stepwise polarity fractions were tested for in vitro inhibition of rat lens AR. Of these, the ethyl acetate (EtOAc) and n-butanol (n-BuOH) fractions exhibited AR inhibitory activities. A chromatographic separation of the active EtOAc and n-BuOH fractions led to the isolation of nine compounds, which were known and identified as vitexin (1), genkwanin-5-O-glucoside (2), vitexin 2″-p-hydroxybenzoate (3), kaempferol-3-O-rutinoside (4), isovitexin (5), quercitrin (6), isoquercitrin (7), 1,5-di-O-caffeoylquinic acid (8), and Vicenin -1 (9). Among these compounds, compounds 6, 7, and 8 showed very strong inhibitory activities against AR (IC50’s = 0.34, 0.32, and 0.08 μM, respectively).
CONCLUSIONS:
The results suggest that S. grandifolia is clearly a potential source for therapeutic agent that might be useful for the treatment of diabetic complications.

Regulatory Toxicology and Pharmacology, 2016:S0273230016303026.

Acute and repeated doses (28 days) oral toxicity study of Vicenin-1, a flavonoid glycoside isolated from fenugreek seeds in laboratory mice.[Reference: WebLink]

Vicenin -1 (fenugreek glycoside) has been proven to possess potent anti-inflammatory and anti-oxidant activity. The objective of the present investigation was to determine in-vivo acute and subacute (28-days repeated dose) oral toxicity of Vicenin -1 isolated from fenugreek seed.
METHODS AND RESULTS:
Vicenin-1 (93%) was isolated from a hydroalcoholic extract of fenugreek seed and characterized using HPLC, TLC, 1H NMR and 13C NMR. Acute oral toxicity (AOT) and subacute toxicity studies of Vicenin -1 were carried out according to OECD 425 (up-and-down procedure) and OCED 407 guidelines in Swiss albino mice. In AOT, Vicenin -1showed 10% mortality when administered at a dose of 5000 mg/kg. However, when vicenin-1 was administered for at doses of 37.5, 75, or 150 mg/kg 28-days it did not show any mortality at the administered doses. Vicenin -1 (75 mg/kg) did not show observational, behavioral, biochemical or histopathological toxic effects. There were minor alterations in body weight, hematology, and histopathology of mice administered with Vicenin -1 (150 mg/kg), but these changes were within normal laboratory ranges. The highest concentration of Venicin-1 was found in liver (3.46%) followed by lung (0.65%).
CONCLUSIONS:
In conclusion, Vicenin -1 showed median lethal dose (LD50) of 4837.5 mg/kg with no-observed-adverse-effect levels (NOAEL) at 75 mg/kg and lowest adverse effect levels (LOAEL) at 150 mg/kg for both sexes of mice during AOT and sub-acute toxicity study, respectively.