(-)-Epigallocatechin-3-(3''-O-methyl) gallate
(-)-Epigallocatechin-3-(3''-O-methyl)gallate shows antioxidative,
cytotoxic, antiallergic, and anti-inflammatory activities, it can negatively regulate basophil activation through the suppression of FcepsilonRI expression. Epigallocatechin-3-(3''-O-methyl)gallate has the function for cold preservation of primary rat hepatocytes.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Agr. Biol. Chem., 2000, 64(10):2218-20.
Antioxidative activity of (-)-epigallocatechin-3-(3''-O-methyl)gallate isolated from fresh tea leaf and preliminary results on its biological activity.[Pubmed:
11129598 ]
Antioxidative activity of (-)-Epigallocatechin-3-(3''-O-methyl) gallate (catechine) was examined.
METHODS AND RESULTS:
Catechine showed a strong antioxidative activity. A preliminary test using rat cancer cells suggests that catechin e also has a strong cytotoxic activity. Among tested catechins, only catechin e has strong activity for both.
J. Agr. Food Chem., 2002, 50(20):5729-34.
Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses FcepsilonRI expression in human basophilic KU812 cells.[Pubmed:
12236706]
We previously found that the O-methylated derivative of (-)-Epigallocatechin-3-O-gallate (EGCg),
(-)-epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG' '3Me), has potent antiallergic activity.
METHODS AND RESULTS:
The high-affinity IgE receptor, FcepsilonRI, is found at high levels on basophils and mast cells and plays a key role in a series of acute and chronic human allergic reactions. To understand the mechanism of action for the antiallergic EGCG' '3Me, the effect of EGCG' '3Me on the cell surface expression of FcepsilonRI in human basophilic KU812 cells was examined. Flow cytometric analysis showed that EGCG' '3Me was able to decrease the cell surface expression of FcepsilonRI. Moreover, immunoblot analysis revealed that total cellular expression of the FcepsilonRI alpha chain decreased upon treatment with EGCG' '3Me. FcepsilonRI is a tetrameric structure comprising one alpha chain, one beta chain, and two gamma chains. The level of mRNA production of each subunit in KU812 cells was investigated. EGCG' '3Me reduced FcepsilonRI alpha and gamma mRNA levels. The cross-linkage of FcepsilonRI causes the activation of basophils, which leads to the secretion of inflammatory mediators including histamine. EGCG' '3Me treatment inhibited the FcepsilonRI cross-linking-induced histamine release.
CONCLUSIONS:
These results suggested that EGCG' '3Me can negatively regulate basophil activation through the suppression of FcepsilonRI expression.
