Daphnenone

J Asian Nat Prod Res. 2012;14(11):1020-6.

Two new compounds with cytotoxic activity on the human melanoma A375-S2 cells from Daphne giraldii callus cells.[Pubmed: 23106433 ]

METHODS AND RESULTS:
Four compounds were isolated from Daphne giraldii callus cells, and their structures were characterized as Daphnenone (1), daphnolon (2), R-( - )-1-(4'-hydroxyphenyl)-3-hydroxy-5-phenyl-1,5-pentandione (3), and S-(+)-daphneolone-4'-O-β-d-glucoside (4) on the basis of MS, NMR spectrographic analysis, and chemical methods. All of the four compounds possessed C6-C5-C6 carbon skeleton, and among them, 3 and 4 were two new compounds.
CONCLUSIONS:
In activity screening test, compounds 1, 2, 3, and 4 showed different degrees of cytotoxic activity against the tumor cells of human melanoma A375-S2 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium hydrobromide method, with IC(50) values of 29.8, 51.0, 41.0, and 150.0 μM, respectively. Furthermore, we found the important target which could explain the cytotoxic mechanism of the four compounds by using autodock 4.0, a structure-guided discovery approach, and the important residues CYS532, GLY534, and SER535 of B-Raf kinase have been discovered.

Nat Prod Res. 2006 Dec;20(14):1290-4.

Two new chemical constituents from Daphne odora Thunb. var. marginata.[Pubmed: 17393653 ]

METHODS AND RESULTS:
Two new phenolic constituents, Daphnenone (1) and daphneone (2), were isolated from the stem bark of Daphne odora Thunb. var. marginata. Their structures were established on the basis of spectroscopic analysis. Compounds 1 and 2 were tested for cytotoxic activity by MTT assays on five human tumour cell lines, K562, A549, MCF-7, LOVO and HepG2.
CONCLUSIONS:
Compound 1 showed obvious cytotoxic activity against all the five cell lines.