Daphnelantoxin B
	
				
				Daphnelantoxin B shows inhibition on aphid body detoxify enzyme system.
				
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			J Biochem Mol Toxicol.2021, 35(5):e22731.
			Applied Biological Chemistry2020, 63:37.
			Front Cell Dev Biol.2020, 8:32.
			J Microbiol Immunol Infect.2021, S1684-1182(21)00142-0. 
			Biomolecules.2021, 11(10):1537.
			Int J Pharm.2022, 618:121636. 
			Biol Pharm Bull.2023, 46(2):245-256.
			Plants (Basel).2021, 10(5):951.
	 
	
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Journal of Sichuan University (Natural Science Edition), 2001. 
EFFECTS OF DAPHNELANTOXIN B ON THE ESTERASES AND THE GLUTATHIONE-S-TRANSFERASE(GST).[Reference: 
WebLink]
The alive body and away body enzyme coped with liquid of the brown citrus aphid(Homoptera: Aphididae) using Daphnelantoxin B with toxic active component. 
METHODS AND RESULTS:
The inhibition of esterase activity on the whole body of the aphid, were evaluated with α and β naphthyl esters using a spectrophotometric assay and native polyacrylanmide gel electrophonesis. And, the inhibition of GST activity on whole body of the aphid, was studied using a spectrophotometric assay. Daphnelantoxin B exhibited higher inhibition of the esterase activity and the GST activity of the aphid regardless of the alive body, away body for enzyme source. But,the study also displayed Daphnelantoxin B was of no significant effect on AChE of the aphid. 
CONCLUSIONS:
The results from Daphnelantoxin B biochemical experiments and subsequent analysis indicate that the inhibition of Daphnelantoxin B on the detoxicification enzyme systems was propitious to further toxic reaction of Daphnelantoxin B in target sit of the aphid body. So, Daphnelantoxin B inhibition on aphid body detoxify enzyme system may be one intoxicant key factor against the aphid.