Daphnelantoxin B
Daphnelantoxin B shows inhibition on aphid body detoxify enzyme system.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Journal of Sichuan University (Natural Science Edition), 2001.
EFFECTS OF DAPHNELANTOXIN B ON THE ESTERASES AND THE GLUTATHIONE-S-TRANSFERASE(GST).[Reference:
WebLink]
The alive body and away body enzyme coped with liquid of the brown citrus aphid(Homoptera: Aphididae) using Daphnelantoxin B with toxic active component.
METHODS AND RESULTS:
The inhibition of esterase activity on the whole body of the aphid, were evaluated with α and β naphthyl esters using a spectrophotometric assay and native polyacrylanmide gel electrophonesis. And, the inhibition of GST activity on whole body of the aphid, was studied using a spectrophotometric assay. Daphnelantoxin B exhibited higher inhibition of the esterase activity and the GST activity of the aphid regardless of the alive body, away body for enzyme source. But,the study also displayed Daphnelantoxin B was of no significant effect on AChE of the aphid.
CONCLUSIONS:
The results from Daphnelantoxin B biochemical experiments and subsequent analysis indicate that the inhibition of Daphnelantoxin B on the detoxicification enzyme systems was propitious to further toxic reaction of Daphnelantoxin B in target sit of the aphid body. So, Daphnelantoxin B inhibition on aphid body detoxify enzyme system may be one intoxicant key factor against the aphid.
