5'-S-Methyl-5'-thioadenosine
S-Methyl-5′-thioadenosine can inhibit the activity of ThiC (4-amino-5-hydroxymethyl-2-
methylpyrimidine phosphate synthase).
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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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J Biol Chem. 2013 Oct 18;288(42):30693-9.
The thiamine biosynthetic enzyme ThiC catalyzes multiple turnovers and is inhibited by S-adenosylmethionine (AdoMet) metabolites.[Pubmed:
24014032 ]
ThiC (4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase; EC 4.1.99.17) is a radical S-adenosylmethionine (AdoMet) enzyme that uses a [4Fe-4S](+) cluster to reductively cleave AdoMet to methionine and a 5'-deoxyadenosyl radical that initiates catalysis. In plants and bacteria, ThiC converts the purine intermediate 5-aminoimidazole ribotide to 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate, an intermediate of thiamine pyrophosphate (coenzyme B1) biosynthesis.
METHODS AND RESULTS:
In this study, assay conditions were implemented that consistently generated 5-fold molar excess of HMP, demonstrating that ThiC undergoes multiple turnovers. ThiC activity was improved by in situ removal of product 5'-deoxyadenosine. The activity was inhibited by AdoMet metabolites S-adenosylhomocysteine, adenosine, 5'-deoxyadenosine, S-methyl-5'-thioadenosine(5'-S-Methyl-5'-thioadenosine), methionine, and homocysteine.
CONCLUSIONS:
Neither adenosine nor S-methyl-5'-thioadenosine(5'-S-Methyl-5'-thioadenosine) had been shown to inhibit radical AdoMet enzymes, suggesting that ThiC is distinct from other family members. The parameters for improved ThiC activity and turnover described here will facilitate kinetic and mechanistic analyses of ThiC.
Zhong Yao Cai. 2013 Oct;36(10):1620-2.
Isolation and structure elucidation of chemical constituents from Pinellia ternata.[Pubmed:
24761673]
To isolate and identify the chemical constituents of ethanol extract of Pinellia ternata.
METHODS AND RESULTS:
The constituents were isolated by silica-gel, Sephadex LH-20 chromatography and HPLC techniques. The structures were identified by spectroscopic analysis including 2D NMR techniques and chemical properties. Nine compounds were obtained and identified as uridine (1), 5'-S-Methyl-5'-thioadenosine (2), adenine (3), chrysophanol (4), 5-hydroxymethylfurfural (5), nicotinamide (6), (2S)-1-O-(9Z, 12Z-octadecadienoyl)-3-O-beta-galactopyranosylglycerol (7), daucosterol (8), beta-sitosterol (9).
CONCLUSIONS:
Compounds 2, 6, 7 are isolated from this plant for the first time.
