Shyobunone
Shyobunone and isoshyobunone have insecticidal and repellant activity, they exhibit strong contact toxicity against Lasioderma serricorne (LS adults with LD50 values of 20.24 and 24.19 ug/adult, respectively; they are strongly repellent against Tribolium castaneum (TC) at 78.63 nL/cm2.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Yao Xue Xue Bao. 2015 Jan;50(1):86-93.
The effective parts of liangxue tongyu prescription on cooling-blood and activating-blood and analysis of chemical constituents by HPLC-MS and GC-MS.[Pubmed:
25924481]
In order to clarify material basis of effective parts of liangxue tongyu prescription, blood-heat and blood-stasis rat model induced by dry yeast was established.
METHODS AND RESULTS:
The changes of rectal temperature, blood viscosity and plasma viscosity were used to evaluate the cooling-blood and activating-blood effects of liangxue tongyu prescription and its parts. Compared with the model group, the extract from liangxue tongyu prescription, its volatile oil and n-butanol part could significantly reduce rectal temperature (P<0.01), and also reduce blood viscosity and plasma viscosity to various degrees (P<0.01 or P<0.05). So volatile oil and n-butanol part were primarily identified as effective parts of liangxue tongyu prescription. By using GC-MS with normalization method of area to analyze volatile oil of liangxue tongyu prescription, 70 compounds were identified, accounting for about 92.54%, mainly as β-asarone, paeonol, α-asarone and Shyobunone. 42 compounds such as peony glycosides, tannins, and iridoid glycosides were identified by HPLC-MS techniques and standard comparison.
CONCLUSIONS:
The study determined the effective parts of liangxue tongyu prescription and clarified the chemical composition providing the foundation for further studies on material basis of liangxue tongyu prescription.
Pharmacogn. Mag., 2015, 11(44):675-81.
Repellant and insecticidal activities of shyobunone and isoshyobunone derived from the essential oil of Acorus calamus rhizomes.[Pubmed:
26600710]
METHODS AND RESULTS:
The main components of the essential oil were identified to be isoShyobunone (15.56%), β-asarone (10.03%), bicyclo[6.1.0]non-1-ene (9.67%), Shyobunone (9.60%) and methylisoeugenol (6.69%). Among them, the two active constituents were isolated and identified as Shyobunone and isoShyobunone. The essential oil showed contact toxicity against LS and TC with LD50 values of 14.40 and 32.55 μg/adult, respectively. The isolated compounds, Shyobunone and isoShyobunone also exhibited strong contact toxicity against LS adults with LD50 values of 20.24 and 24.19 μg/adult, respectively, while the LD50 value of isoShyobunone was 61.90 μg/adult for TC adults.
CONCLUSIONS:
The essential oil, Shyobunone and isoShyobunone were strongly repellent (98%, 90% and 94%, respectively, at 78.63 nL/cm(2), after 2 h treatment) against TC.
