Schisanhenol

Fitoterapia. 2015 Mar;101:117-24.

Schisanhenol derivatives and their biological evaluation against tobacco mosaic virus (TMV).[Pubmed: 25598185]

Schisanhenol (Sol) was isolated from Schisandra rubriflora, and a series of derivatives (1-16, 15a-16a, and 15b-16b) were designed and prepared by chemical modification. The curative and protective effects of these dibenzocyclooctadiene lignan analogues against tobacco mosaic virus (TMV) were evaluated.
METHODS AND RESULTS:
Most analogues exhibited stronger protective effects than the positive control ningnanmycin. DibromoSchisanhenol (6) at 0.25mM exhibited the strongest protective activity (83.5±1.8% at 0.25mM), and 14-(3, 5-dibenzyloxy)-benzoyloxySchisanhenol (16) showed a significant curative effect (78.0±3.8% at 0.15mM) that was much stronger than that of the commercial virucide ningnanmycin.
CONCLUSIONS:
This study is the first to demonstrate that natural dibenzocyclooctadiene lignans and analogues are active against plant viruses.

Biomed Environ Sci. 1992 Mar;5(1):57-64.

Protective effect of schisanhenol against oxygen radical induced mitochondrial toxicity on rat heart and liver.[Pubmed: 1586468]

Schisanhenol (SAL) had been shown to have potent antioxidant activities. The protective effects of SAL against oxygen radical induced mitochondrial injuries of rat heart and liver were investigated in the present study.
METHODS AND RESULTS:
The ferrous-cysteine induced mitochondrial lipid peroxidation was significantly inhibited by SAL, while the loss of ATPase activity induced by the lipid peroxidation was prevented. The rigidification of mitochondrial membrane, as well as swelling, lysis and disintegration of the mitochondria induced by ferrous-cysteine were all significantly inhibited by SAL.
CONCLUSIONS:
These results further confirm that SAL possesses antioxidant activity.

Sheng Li Ke Xue Jin Zhan. 1998 Jan;29(1):35-8.

Protective effects of schisanhenol, salvianolic acid A and SY-L on oxidative stress induced injuries of cerebral cells and their mechanisms.[Pubmed: 12501701]

Oxidative stress may play an important role in neuronal degenerative diseases such as Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis. These disorders make the elderly not being able to live normally and move freely. So it is necessary to find effective antioxidants to prevent or cure the aged persons from diseases related to neuronal degeneration.
METHODS AND RESULTS:
Schisanhenol (Sal) and salvianolic acid A (Sal A) are known antioxidants which were isolated from Chinese herbs respectively. SY-L is a totally synthetic new compound.
CONCLUSIONS:
The results showed that Sal, Sal A and SY-L significantly protect cerebral cells from the injuries induced by oxidative stress.

Acta Pharmacol Sin. 2004 Aug;25(8):1038-44.

Antioxidative effect of schisanhenol on human low density lipoprotein and its quantum chemical calculation.[Pubmed: 15301737]

To investigate the effect of Schisanhenol (Sal) on copper ion-induced oxidative modulation of human low density lipoprotein (LDL).
METHODS AND RESULTS:
The antioxidative activity of eight schisandrins (DCL) on microsome lipid peroxidation induced by Vit C/NADPH system was first observed, and then, the effect of Sal on Cu2+-induced human LDL oxidation was studied. The generation of malondialdehyde (MDA), lipofuscin, reactive oxygen species (ROS), consumption of a-tocopherol as well as electrophoretic mobility of LDL were determined as criteria of LDL oxidation. Finally, the quantum chemical method was used to calculate the theoretical parameters of eight DCL for elucidating the difference of their antioxidant ability. Sal was shown to be the most active one among eight schizandrins in inhibiting microsome lipid oxidation induced by Vit C/NADPH. Sal 100, 50, and 10 micromol/L inhibited production of MDA, lipofuscin and ROS as well as the consumption of a-tocopherol in Cu2+-induced oxidation of human LDL in a dose-dependent manner. Sal also reduced electrophoretic mobility of the oxidized human LDL. Further study of quantum chemistry found that Sal was the strongest one among eight DCL to scavenge O2, R, RO and ROO radicals.
CONCLUSIONS:
Sal has antioxidative effect on human LDL oxidation. The mechanism of Sal against LDL oxidation may be through scavenging free radicals.

J Asian Nat Prod Res. 2008 Jul-Aug;10(7-8):799-806.

Schisanhenol attenuated ox-LDL-induced apoptosis and reactive oxygen species generation in bovine aorta endothelial cells in vitro.[Pubmed: 18696334]

The aim of this paper was to investigate the protective effect of Schisanhenol (Sal) isolated from Schisandra rubriflora Rhed, on human ox-LDL-induced bovine aorta endothelial cells (BAECs) apoptosis and intracellular reactive oxygen species (ROS) production in vitro.
METHODS AND RESULTS:
The BAECs were cultured with ox-LDL (200 microg/ml) in the presence and absence of Sal (10 and 50 micromol L(- 1)) for 24 h. The cytotoxicity of ox-LDL was evaluated by LDH leakage, cell viability and morphological change. Cell apoptosis was estimated by DNA ladder, chromatin condensation, and flow cytometry assay. The intracellular ROS production was detected by using DCF, a ROS probe, with laser confocal microscopy and flow cytometry. Sal was shown to reduce LDH leakage and increase cell viability. Sal also attenuated ox-LDL-induced BAECs apoptosis as indicated in typical internucleosomal DNA degradation (DNA ladder), condensed chromatin, and the sub-G1 peak appearance in flow cytometry assay. Furthermore, Sal was shown to inhibit ROS generation in BAECs with stimulation of ox-LDL.
CONCLUSIONS:
The results indicated that the anti-apoptosis effect of Sal on BACSs might be related to its inhibition of ROS generation.