Ecdysterone 20,22-monoacetonide
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Phytoecdysteroids ofRhaponticum carthamoides.[Reference:
WebLink]
METHODS AND RESULTS:
From the roots with rhizomes of the plantRhaponticum carthamoides Willd) Iljin Compositae), in addition to integristerone A, ecdysterone, polypodin B, 2-deoxyecdysterone, and 24(28)-dehydromakisterone A, we have isolated the new compounds ecdysteron3–2,3-monoacetonide (I), Ecdysterone 20,22-monoacetonide (II)) and rhapisterone (III): I — C30H48O7, mp 232–233° (ethyl acetate-methanol) [α] D20 +56.4±2° (c 0.0; methanol); II — C30H48O7, mp 227–229° (ethyl acetate-methanol), [α] D20 +60.1±2° (c 1.3; methanol); III — C29H48O7, mp, 241–242° (ethyl acetate-methanol), [α] D20 +30±2° (c 0.1; dioxane).
CONCLUSIONS:
The structure of (III) was established on the basis of spectral characteristics as 2β, 3β, 14α, 20R, 22R, 23ζ-5β-stigmast-7-en-6-one. Details of the PMR, mass, and IR spectra of all the compounds and of the CD of rhapisterone are given.
