Daphniyunnine B
Daphniyunnine B is a natural product from Daphniphyllum macropodum Miq.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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J Nat Prod. 2006 Apr;69(4):553-7.
Daphniyunnines A-E, alkaloids from Daphniphyllum yunnanense.[Pubmed:
16643024 ]
METHODS AND RESULTS:
The first chemical study on the stems and leaves of Daphniphyllum yunnanense led to the isolation of five new alkaloids, Daphniyunnine A, Daphniyunnine B, daphniyunnine C, daphniyunnine D, daphniyunnine E (1-5). Daphniyunnines B-E (2-5) are four unusual C-22 nor-Daphniphyllum alkaloids.
CONCLUSIONS:
The structures of these alkaloids were characterized by spectroscopic methods, especially 2D NMR techniques. A single-crystal X-ray diffraction analysis was used to confirm the structure of 1. Daphniyunnine D (4) showed cytotoxicity against two tumor cell lines, P-388 and A-549, with IC(50) values of 3.0 and 0.6 microM, respectively.
