Curzerene

J Egypt Soc Parasitol. 2010 Dec;40(3):699-706.

Efficacy of volatile oils (curzerene, furanoeudesma-1, 3-diene and lindestrene) on avian coccidiosis under laboratory conditions.[Pubmed: 21268538]

METHODS AND RESULTS:
The coccidicidal efficacy of volatile oils (Curzerene, furanoeudesma-1, 3-diene and lindestrene) against unsporulated and sporulated chicken Eimeria species oocysts was tested in three concentrations: 1, 2 & 3 microg/ml. Marked reduction in the number of living oocysts was recorded in exposed groups. The concentration of 3 microg/ml volatile oils induced the highest destructive effect. 58.1% of viable unsporulated oocysts were destroyed.
CONCLUSIONS:
A mean number of 153,800 oocysts was the difference between the total number of the produced oocysts per gram faeces in the group infected with exposed oocysts and that of the group infected with non exposed oocysts being less in the exposed group with more reduction in the vitality of shedding oocysts in the former group. At the meantime, the postmortem and histopathological microscopical examination of the intestine and caecum of the tested group revealed a reduction in the intestinal lesions in the group infected with the exposed oocysts.

Planta Med. 2017 Jan;83(1-02):23-29.

Cytotoxic and Antitumor Effects of Curzerene from Curcuma longa.[Pubmed: 27286338]

Curzerene is a sesquiterpene and component used in oriental medicine. It was originally isolated from the traditional Chinese herbal medicine Curcuma rhizomes.
METHODS AND RESULTS:
In this study, anticancer activity of Curzerene was examined in both in vitro and in vivo models. The result of the MTT assay showed that Curzerene exhibited antiproliferative effects in SPC-A1 human lung adenocarcinoma cells in a time-dependent and dose-dependent manner. The anticancer IC50s were 403.8, 154.8, and 47.0 μM for 24, 48, and 72 hours, respectively. The flow cytometry analysis indicated Curzerene arrested the cells in the G2/M cell cycle and promoted or induced apoptosis of SPC-A1 cells. The percentage of cells arrested in the G2/M phase increased from 9.26 % in the control group cells to 17.57 % in the cells treated with the highest dose (100 μM) of Curzerene. Western blot and RT-PCR analysis demonstrated that Curzerene induced the downregulation of GSTA1 protein and mRNA expressions in SPC-A1 cells. Tumor growth was significantly inhibited in SPC-A1 cell-bearing nude mice by using Curzerene (135 mg/kg daily), meanwhile, Curzerene did not significantly affect body mass and the organs of the mice, which may indicate that Curzerene has limited toxicity and side effects in vivo.
CONCLUSIONS:
In conclusion, Curzerene could inhibit the proliferation of SPC-A1 human lung adenocarcinoma cells line in both in vitro and in vivo models. Focusing on its relationship with GSTA1, Curzerene could induce the downregulation of GSTA1 protein and mRNA expressions in SPC-A1 cells. Curzerene might be used as an anti-lung adenocarcinoma drug candidate compound for further development.

Fitoterapia. 2014 Sep;97:133-41.

Wild celery (Smyrnium olusatrum L.) oil and isofuranodiene induce apoptosis in human colon carcinoma cells.[Pubmed: 24924290]

Smyrnium olusatrum (Apiaceae), well known as wild celery, is a biennal celery-scented plant used for many centuries as a vegetable, then abandoned after the introduction of celery.
METHODS AND RESULTS:
In the present work, the essential oil obtained from inflorescences and the amounts of its main constituents isofuranodiene, Curzerene and germacrone were analyzed by GC as well as by HPLC because of their degradation (Cope rearrangement) occurring at high temperatures. The oil and the main constituents were assayed for cytotoxic activity on the human colon cancer cell line (HCT116) by MTT assay. Flower oil and isofuranodiene showed noteworthy activity on tumor cells with IC50 of 10.71 and 15.06 μg/ml, respectively.
CONCLUSIONS:
Analysis of the cytotoxic activity showed that wild celery oil and isofuranodiene are able to induce apoptosis in colon cancer cells in a time and concentration-dependent manner suggesting a potential role as models for the development of chemopreventive agents.

Chinese Arch. Trad. Chinese Med., 2009(12):2496-9.

Study of Curzerene Curing the Animal Model Of Breast Precancer Rats induced by DMBA.[Reference: WebLink]

To research the effect of treating breast precancer with Curzerene by observed in Pathological Changes.
METHODS AND RESULTS:
256 SD rats were randomly divided into seven groups:the blank control group,the breast precancer model group,the comparative group of kanglaite,the comparative group of tamoxifen,large middle and small remedial groups of Curzerene,Animal model of rat breast precancer was induced with DMBA.Interpose treatment was performed the second day,effect and analysis of pathology the 8th 10th 12th and 14th week. Compared with the blank control group,the rate of death of the breast precancer model group increased(P0.05),the probability of cancer,precancer and death of the middle and small remedial groups of Curzerene decreased, Compared with the breast precancer model group had significant difference(P0.05),Compared with group of tamoxifen hadn't significant difference(P0.05).
CONCLUSIONS:
Curzerene could cure and prevent breast precancer.