Calystegine B4
Calystegine B4 inhibits almond beta-glucosidase and pig kidney trehalase in a competitive manner, with a Ki value of 7.3 microM and 1.2 microM, respectively.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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J Agric Food Chem. 2013 Jun 19;61(24):5893-902.
Glycoalkaloid and calystegine levels in table potato cultivars subjected to wounding, light, and heat treatments.[Pubmed:
23692427]
Potato tubers naturally contain a number of defense substances, some of which are of major concern for food safety. Among these substances are the glycoalkaloids and calystegines.
METHODS AND RESULTS:
We have here analyzed levels of glycoalkaloids (α-chaconine and α-solanine) and calystegines (A₃, B₂, and B₄) in potato tubers subjected to mechanical wounding, light exposure, or elevated temperature: stress treatments that are known or anticipated to induce glycoalkaloid levels. Basal glycoalkaloid levels in tubers varied between potato cultivars. Wounding and light exposure, but not heat, increased tuber glycoalkaloid levels, and the relative response differed among the cultivars. Also, calystegine levels varied between cultivars, with Calystegine B4 showing the most marked variation. However, the total calystegine level was not affected by wounding or light exposure.
CONCLUSIONS:
The results demonstrate a strong variation among potato cultivars with regard to postharvest glycoalkaloid increases, and they suggest that the biosynthesis of glycoalkaloids and calystegines occurs independently of each other.
Carbohydr Res. 1996 Oct 31;293(2):195-204.
Calystegine B4, a novel trehalase inhibitor from Scopolia japonica.[Pubmed:
8938376]
We gave this new alkaloid the trivial name Calystegine B4.
METHODS AND RESULTS:
The structure of Calystegine B4 was determined as 1 alpha, 2 beta, 3 alpha, 4 alpha-tetrahydroxy-nor-tropane from a variety of NMR spectral data. Calystegines B1, B2, and C1 are potent competitive inhibitors with Ki values ranging from 10(-6) to 10(-7) M for almond beta-glucosidase, while Calystegine B4 inhibited this enzyme in a competitive manner, with a Ki value of 7.3 microM. Calystegine B2 is also a potent inhibitor of green coffee bean alpha-galactosidase, whereas Calystegine B4 exhibited no significant activity for this enzyme.
CONCLUSIONS:
Among rat intestinal glycosidases, only trehalase was potently inhibited by Calystegine B4, with an IC50 value of 9.8 microM. Furthermore, Calystegine B4 potently inhibited pig kidney trehalase in a competitive manner, with a Ki value of 1.2 microM, but it was almost inactive against yeast and fungal trehalases.
