(-)-Bicuculline methochloride
(-)-Bicuculline methochloride is a GABAA-receptor antagonist. Exposure to 100 microM (-)-Bicuculline methochloride for 48 hr can result in prominent CA1 cell death.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
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Int J Neurosci. 2010 Dec;120(12):752-9.
Effect of low Mg2+ and bicuculline on cell survival in hippocampal slice cultures.[Pubmed:
20942591]
A reliable model system of epileptiform insult would facilitate investigation into the underlying biological mechanisms.
METHODS AND RESULTS:
Epileptiform insult was induced in hippocampal slice cultures by lowering extracellular Mg(2+), (+)-bicuculline, or (-)-Bicuculline methochloride, a stable salt form of bicuculline (both forms block GABA(A) receptors). Cell death was assessed by propidium iodide uptake. Low Mg(2+) or (+)-bicuculline did not produce cell death regardless of dose or incubation period. Exposure to 100 microM (-)-Bicuculline methochloride for 48 hr resulted in prominent CA1 cell death.
CONCLUSIONS:
These findings demonstrate that not all pro-epileptic drugs/ion changes used routinely for electrophysiological recording of seizure activity lead to cell death in hippocampal slice cultures and that treatment with bicuculline methochloride can be used as a reliable model for epileptiform insult.
