(-)-Bicuculline methochloride
(-)-Bicuculline methochloride is a GABAA-receptor antagonist. Exposure to 100 microM (-)-Bicuculline methochloride for 48 hr can result in prominent CA1 cell death.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Int J Neurosci. 2010 Dec;120(12):752-9.
Effect of low Mg2+ and bicuculline on cell survival in hippocampal slice cultures.[Pubmed:
20942591]
A reliable model system of epileptiform insult would facilitate investigation into the underlying biological mechanisms.
METHODS AND RESULTS:
Epileptiform insult was induced in hippocampal slice cultures by lowering extracellular Mg(2+), (+)-bicuculline, or (-)-Bicuculline methochloride, a stable salt form of bicuculline (both forms block GABA(A) receptors). Cell death was assessed by propidium iodide uptake. Low Mg(2+) or (+)-bicuculline did not produce cell death regardless of dose or incubation period. Exposure to 100 microM (-)-Bicuculline methochloride for 48 hr resulted in prominent CA1 cell death.
CONCLUSIONS:
These findings demonstrate that not all pro-epileptic drugs/ion changes used routinely for electrophysiological recording of seizure activity lead to cell death in hippocampal slice cultures and that treatment with bicuculline methochloride can be used as a reliable model for epileptiform insult.
