7beta-Hydroxybufalin

Structure
7beta-Hydroxybufalin
Summary
Product Name 7beta-Hydroxybufalin
CAS No.: 20143-97-9
Catalog No.: CFN94058
Molecular Formula: C24H34O5
Molecular Weight: 402.53 g/mol
Purity: >=98%
Type of Compound: Steroids
Physical Desc.: Powder
Source: The glandular body of Bufo bufo gargarizans Cantor
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Price:
Description
Reference standards.
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Storage
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Product Use Citation
  • Int J Mol Sci.202, 25(17):9246.
  • Plant Sci.2021, 313:111069.
  • Industrial Crops and Products2023, 199:116746.
  • Phytomedicine Plus2022, 2(1):100207.
  • Biomed Pharmacother.2022, 145:112410.
  • Comparative Clinical Pathology 2021, 30:961-971.
  • J Sci Food Agric.2023, 103(1):213-220.
  • Natural Product Communications2021, 16(9):1-10.
  • J Control Release.2024, 375:300-315.
  • Eur J Pharmacol.2024, 978:176800.
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    Structure Identification
    J Nat Prod. 2005 Apr;68(4):626-8.
    Microbial hydroxylation of bufalin by Cunninghamella blakesleana and Mucor spinosus.[Pubmed: 15844967 ]

    METHODS AND RESULTS:
    The microbial transformation of a cytotoxic bufadienolide, bufalin (1), was carried out using two strains of filamentous fungi. Cunninghamella blakesleana catalyzed the specific 12alpha-hydroxylation of bufalin and produced 12alpha-hydroxybufalin (2) and 7beta,12alpha-dihydroxybufalin (3) as the major metabolites, together with 7beta-Hydroxybufalin (4) and 12beta-hydroxybufalin (5) in low yields. Two minor products were isolated from the culture broth of Mucor spinosus and were identified as 7beta,15alpha-dihydroxybufalin (6) and 5beta,7beta-dihydroxybufalin (7), respectively. Metabolites 2, 3, 6, and 7 are new compounds, and their structures were fully characterized by NMR and MS spectroscopy.
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