5-Hydroxy-7-acetoxyflavone
5-Hydroxy-7-acetoxyflavone is a natural product from Oroxylum indicum.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Analysis on chemical constituents in Bupleuri Radix by HPLC-Q-TOF-MS[Reference:
WebLink]
To investigate the chemical constituents in extract of Bupleuri Radix using HPLC-Q-TOF-MS.
METHODS AND RESULTS:
The HPLC separation achieved on Agilent 1200 HPLC Diamonsil Ⅱ C_(18) column(250 mm × 4.6 mm,5μm) was used with a mobile phase of0.05%H_2O-formic acid(A) and acetonitrile(B),the flow rate was 1.0 mL/min,the column temperature was 35℃,and the detection wavelength was 200-600 nm.Positive and negative ions MS information and element analysis,via comparing MS data with those of the standard compounds and coupled with the related literature were used to analyze the main chemical constituents of BupleuriRadix. Twenty-one chemical constituents were identified,including three phenolic acid,four flavonoids,and fourteen triterpenoid saponins,and isochlorogenic acid A(5),isochlorogenic acid B(6),7,3'-di-O-methylquercetin(8),and 5-Hydroxy-7-acetoxyflavone(24) were separated from Bupleuri Radix for the first time.
CONCLUSIONS:
It is used to quickly analyze the chemical constituents from Bupleuri Radix by establishing a rapid accurate evaluation method using HPLC-Q-TOF-MS,which could provide the references for the application development.