10-O-Methylprotosappanin B

10-O-Methylprotosappanin B
Product Name 10-O-Methylprotosappanin B
CAS No.: 111830-77-4
Catalog No.: CFN96304
Molecular Formula: C17H18O6
Molecular Weight: 318.3 g/mol
Purity: >=98%
Type of Compound: Phenols
Physical Desc.: Powder
Source: The dried heartwoods of Caesalpinia sappan L.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Price:
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    We have been further studying the isolation, absolute stereochemistries and biosyntheses of the phenolic constituents of C. sappan and C. japonica.
    METHODS AND RESULTS:
    In the present paper,we report the isolation and structural elucidation of a novel dibenzoxocin derivative,10-O-Methylprotosappanin B(1), from Sappan Lignum.10-O-Methylprotosappanin B (1) was obtained by repeated silica gel and Sephadex LH-20 column chromatography, and preparative thin-layer chromatography (PTLC) of the remaining fractions after separation of the previously reported compounds.4) The isolation of the intact compound was so difficult that only a limited amount could be obtained. However, a satisfactory amount was obtained in the form of an isopropylidene derivative (6) from the methanolic extract separated after treatment with acetone in the presence of an acid catalyst, as described in the previous paper.4c) The remaining fractions after isolation of the isopropylidene derivatives of 3,4-dihydroxy homoisoflavans4c) were subjected to column chromatography on silica gel and Sephadex LH-20 to yield 6, together with the isopropylidene derivative of protosappanin B (7). On acid hydrolysis with 60% AcOH, compound 6 was readily transformed into 1.
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