1-Hydroxymethyl-beta-carboline glucoside | CAS:1408311-12-5

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Simultaneous determination of 4 alkaloids and a flavonoid in Picrasmae Ramulus et Folium by RP-HPLC.[Pubmed: 25095379]

METHODS AND RESULTS:
A RP-HPLC method was developed to evaluate the quality of Picrasmae Ramulus et Folium by simultaneous determination of five constituents including 1-hydroxymethyl-beta-carboline (1), 1-methoxicabony-beta-carboline (2), 4-methoxy-5-hydroxy-canthin-6-one (3), 4, 5-dimethoxy-canthin-6-one (4) and maackiain (5) in Picrasmae Ramulus et Folium. The samples were separated on a Kromasil RP-C18 (4.6 mm x 250 mm, 5 microm) column eluted with acetonitrile and 0.1% phosphoric acid as mobile phases in gradient mode. The detection wavelength was set at 254 nm. The calibration curves and linearity of the above five standards were determined as (1) Y = 6 525.6X + 37.25 (0.009-1.780 microg, r = 0.996 8), (2) Y = 3 662.3X + 41.55 (0.005-0.920 microg, r = 0.999 5), (3) Y = 3763.1X + 146.87 (0.015-3.060 microg, r = 0.999 0), (4) Y = 2 174.1X + 21.52 (0.003-0.620 microg, r = 0.999 5), and (5) Y = 276.25X + 7.65 (0.010-1.960 microg, r = 0.998 9), respectively.
CONCLUSIONS:
The method is simple and repeatable, and can be used for the quality assessment of Picrasmae Ramulus et Folium.

Product Name	1-Hydroxymethyl-beta-carboline glucoside
CAS No.:	1408311-12-5
Catalog No.:	CFN96720
Molecular Formula:	C₁₈H₂₀N₂O₆
Molecular Weight:	360.36 g/mol
Purity:	>=98%
Type of Compound:	Alkaloids
Physical Desc.:	Powder
Source:	The plants of Picrasmae Ramulus et Folium.
Solvent:	DMSO, Pyridine, Methanol, Ethanol, etc.
Price: