Sennoside A1
Senna leaf is widely consumed as tea to treat constipation or to aid in weight loss, sennoside A, sennoside A1 , and sennoside B are dirheinanthrone glucosides that are abundant and the bioactive constituents in the plant.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Journal of Separation Science, 2015, 38(20):3502-3507.
Simultaneous separation of three isomeric sennosides from senna leaf (Cassia acutifolia) using counter-current chromatography.[Reference:
WebLink]
Senna leaf is widely consumed as tea to treat constipation or to aid in weight loss. Sennoside A, Sennoside A1 , and Sennoside B are dirheinanthrone glucosides that are abundant and the bioactive constituents in the plant. They are isomers that refer to the (R*R*), (S*S*), and (R*S*) forms of protons on C-10 and C-10' centers and it is difficult to refine them individually due to their structural similarities.
METHODS AND RESULTS:
The new separation method using counter-current chromatography successfully purified sennoside A, A1 , and B from senna leaf (Cassia acutifolia) while reversed-phase medium-pressure liquid chromatography yielded sennoside A only. n-Butanol/isopropanol/water (5:1:6, v/v/v) was selected as the solvent system for counter-current chromatography operation, and the partition coefficients were carefully determined by adding different concentrations of formic acid.
CONCLUSIONS:
High-resolution mass spectrometry and NMR spectroscopy were performed to verify the chemical properties of the compounds.
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