Rotundine

Chinese Journal of Drug Abuse Prevention & Treatment, 2006, 12(5):270-1.

Therapeutic Effects of Rotundine Combined with Methadone in Treatment of Heroin Dependence[Reference: WebLink]

To explore the therapeutic effects of Rotundine combined with methadone in treatment of heroin dependence.
METHODS AND RESULTS:
Methods: Sixty heroin addicts were randomly divided into two groups. Group A were treated with Rotundine plus methadone and group B were treated with methadone alone. Results: The effective rate of group A was significantly higher than that of group B (96.67% and 73.33%, respectively, P 0.05). The total dose of methadone and the days of treatment in group A (275±21.2 mg and 8±2.3 d, respectively) was significantly lower than that of group B (415±31.2 mg and 12±2.5 d, respectively, P 0.01 and P 0.05, respectively).
CONCLUSIONS:
Conclusion: Rotundine combined with methadone treatment may be a better therapeutic method in treatment of heroin dependence.

Journal of Emergenoy Medicine, 2000, 9(6):379-81.

Influence of rotundine on NO、ET-1 and energy metabolism after acute brain injury resulted from complete cerebral ischemia reperfusion in the rats[Reference: WebLink]

METHODS AND RESULTS:
To find the influence of Rotundine(L THP) on NO、ET 1、ATP and Lactate in rat brain tissues after the global ischemia reperfusion and to investigate protective effects of L THP.Methods:The four vessel occlusion model was made as described by Pulsinelli Brierlog in Sprange Daweley rats.The influences of L THP on NO、ET 1 and energy metabolism during the reperfusion period was observed.Results:L THP can suppress the marked increase of NO、ET 1 and lactate in the early stage of reperfusion,and also improve the contents of ATP.
CONCLUSIONS:
L THP provides remarkable protection against cerebral ischemia reperfusion damage.Its mechanisms may be through its influence on NO、ET 1 and energy metabolism.

Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Jun;19(6):361-4.

Effects of rotundine injection on nitric oxide synthase in tissues of different organs after ischemia/reperfusion of brain in rats.[Pubmed: 17577446]

To explore the protective effect of Rotundine injection on lung, liver and kidney damages after cerebral ischemia/reperfusion (I/R) injury in rats based on the activity changes of nitric oxide synthase (NOS).
METHODS AND RESULTS:
Seventy-six rats were randomly divided into three groups: sham operation group, I/R injury group and treatment group, and determinations were done at five different time points. The cerebral I/R models were reproduced by improved 4 vessels occlusion method. The activities of NOS in the lung, liver and kidney were measured in all the rats at 2, 6, 12, 24 and 48 hours after reperfusion. Compared with sham operation group, the activities of total NOS (tNOS) were significantly increased at 2, 12 and 24 hours in I/R injury group (P<0.05 or P<0.01), with the peak value at 12 hours (all P<0.01). The activities of constitutive NOS (cNOS) were increased significantly at 2 hours (all P<0.05), and those of induced NOS (iNOS) were increased at 12 hours (all P<0.01). The activities of iNOS were still high at 24 hours (all P<0.05), and approached the levels of sham operation group at 48 hours. Compared with I/R injury group, the activities of cNOS in various organs increased much higher at 2 hours in treatment group (all P<0.05). But those of iNOS were significantly decreased after 12 hours (P<0.05 or P<0.01).
CONCLUSIONS:
The various types of NOS play different roles in the lung damages after brain I/R injury at different stages in rats. Rotundine injection can ameliorate the damages by modulating the activities of different types of NOS.

Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Nov;34(11):2989-93.

IR and Raman spectra studies of Rotundine based on DFT.[Pubmed: 25752044]

Infrared spectroscopy (IR), the normal Raman spectroscopy (NRS) and the surface enhanced Raman spectroscopy (SERS) in new Ag/Cu nanomaterial of Rotundine were studied in the present paper.
METHODS AND RESULTS:
The IR and the NRS of Rotundine were calculated by the density functional theory (DFT) using B3LYP/6-311+G(d, p), then the spectral intensity graph of Rotundine were given. The vibrational peaks were assigned comprehensively by the visualization software of Gauss view 5. 0. Rotundine has obvious infrared and Raman vibrational peak in the wave number range of 3 300-2500 and 1 800-600 cm(-1). SnCl2 and PVP was used as capping agent for the silver nanoparticles in SERS of Rotundine. Finally, by using the method of cyclic immersion well dispersed silver nanoparticles was obtained and achieved good enhancement effect. This molecule acquired strong selective enhancement vibration peak, In the wave number ranges of 1 500-1 400 and 1 000-700 cm(-1) the enhancement effect is most obvious. After analyzed, the methylene of this molecule is adsorbed on the silver nanoparticles surface and the angle between the benzene ring and the silver substrate is close to 90 degrees. The theoretically calculated spectra of Rotundine are consistent with the obtained experimental spectra. There are some differences may be due to the interaction forces between molecules and so on. The visualization software displayed the structure characteristics and molecular group vibration of this molecular visually and provided important basis for assigning the vibrational peaks.
CONCLUSIONS:
Rotundine is an important traditional Chinese medicine agent contained in many kinds of sedative drugs. The study provides a strong basis for the rapid, feature and trace identification of Rotundine and also supplies important reference for the biological role of central inhibition of analgesic drugs.