Periglaucine B
Periglaucine B can inhibit hepatitis B virus (HBV) surface antigen (HBsAg) secretion in Hep G2.2.15 cells.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Key Engineering Materials2022, 931(47-53).
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Molecules.2023, 28(3):1313.
The Journal of Internal Korean Medicine2015, 36(4):486-497
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Pharmacogn Mag.2015, 11:S585-91
Int J Mol Sci.2021, 22(10):5181.
Molecules 2022, 27(3),1047.
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J Nat Prod. 2008 May;71(5):760-3.
Periglaucines A-D, anti-HBV and -HIV-1 alkaloids from Pericampylus glaucus.[Pubmed:
18396905]
METHODS AND RESULTS:
Four new hasubanane-type alkaloids, periglaucine A, Periglaucine B, periglaucine C, periglaucine D (1-4), and three known alkaloids, norruffscine (5), (-)-8-oxotetrahydropalmatine (6), and (-)-8-oxocanadine (7), were isolated from the aerial parts of Pericampylus glaucus. Their structures were elucidated on the basis of extensive NMR and EIMS data, and that of periglaucine A (1) was confirmed by single-crystal X-ray diffraction.
CONCLUSIONS:
Alkaloids 1-4 inhibited hepatitis B virus (HBV) surface antigen (HBsAg) secretion in Hep G2.2.15 cells.
(-)-8-Oxotetrahydropalmatine (6) possessed a high selectivity index (SI = 22.4) for HBsAg secretion of the Hep G2.2.15 cell line with an IC(50) value of 0.14 mM. Norruffscine (5) and (-)-8-oxotetrahydropalmatine (6) exhibited inhibitory activity against human immunodeficiency virus (HIV-1) with EC(50) values of 10.9 and 14.1 microM in C8166 cells (SI = 45.7 and 18.8), respectively.
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