Huzhangoside B

Biochemical Systematics & Ecology, 2010, 38(5):1018-1025.

Distribution of the triterpenoid saponins and chemotaxonomy of the genus Clematis L. by high-performance liquid chromatography–mass spectrometry.[Reference: WebLink]

METHODS AND RESULTS:
Selected species of the genus Clematis (Ranunculaceae) have been screened for occurrence of triterpenoid saponins by qualitative HPLC-MSn analysis of root and rhizome materials from 18 Clematis samples as well as the whole plant materials of Clematis puberula var. ganpiniana and Clematis terniflora. The HPLC-MSn analysis allowing the detection of 17 oleanolic acid or hederagenin saponins was carried out in the negative selected ion monitoring (SIM) mode. Triterpenoid saponin profiles of these taxa were used for phylogenetic studies, and results are presented as a dendrogram.
CONCLUSIONS:
Huzhangoside B could be unambiguously identified in all analyzed Clematis taxa, as well as in the investigated Ranunculus taxa. The distribution and chemotaxonomic importance of the triterpenoid saponin profile within this genus are discussed.

Chem Pharm Bull (Tokyo). 1998 Dec;46(12):1891-900.

Studies on the constituents of Clematis species. VII. Triterpenoid saponins from the roots of Clematis terniflora DC. var. robusta Tamura.[Pubmed: 9880908]

METHODS AND RESULTS:
From the roots of Clematis terniflora, nine new oleanolic acid 3,28-O-bisdesmosides called clematernosides A, B, E, F, G, H, I, J and K, and two new hederagenin 3,28-O-bisdesmosides called clematernosides C and D, have been isolated together with two known saponins, Huzhangoside B and clematichinenoside C. The structures of the new saponins have been elucidated based on chemical and spectral evidence.
METHODS AND RESULTS:
Among the new spaonins, clematernosides I and J have a nonasaccharide moiety and a total of twelve monosaccharide moieties in the molecule. This is the first report of the isolation and structure elucidation of such "big" glycosides.