Estriol 3-glucuronide
Estradiol-3-glucuronide, estradiol-17beta-glucuronide, estriol-3-glucuronide , estrone
glucuronide, estriol-16alpha-glucuronide, and pregnanediol-3alpha-glucuronide are immunoreactive metabolites of the major urinary estrogens, they have used to ovulation detection methods.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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J Steroid Biochem. 1984 Feb;20(2):621-6.
Preparation of specific antisera to estrone-3-glucuronide and estriol-3-glucuronide.[Pubmed:
6323874]
The preparation and antigenic properties of estrone 3-glucuronide- and Estriol 3-glucuronide-bovine serum albumin conjugates in which the hapten is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-6 position on the steroid nucleus, have been described.
METHODS AND RESULTS:
Antibodies raised against the two immunogens in the rabbit possessed high specificity to estrone 3-glucuronide and Estriol 3-glucuronide, respectively, exhibiting little cross-reactivities with other estrogen conjugates and no cross-reactions with related steroids except for free estrogens, their 3-methyl ethers and 3-sulfates. The cross-reactive antibodies were eliminated by partial immunoadsorption on affinity chromatographic media using the estrone-3-methyl ether 17-(O-carboxymethyl)oxime- and estriol-3-methyl ether 16 (or 17)-hemisuccinate-aminohexyl Sepharose conjugates, respectively.
CONCLUSIONS:
The purified antisera exhibited no cross-reactivities with free estrogens and ring A conjugates of estrone and estriol.
Steroids, 1968, 12(6):717-723.
Isolation of crystalline estriol-3-glucuronide from human pregnancy urine[Pubmed:
4302332]
METHODS AND RESULTS:
Crystalline Estriol 3-glucuronide has been isolated and characterized, as the intact conjugate, from human pregnancy urine. The infrared spectrum of the isolated conjugate was identical with that of a synthetic sample. Enzymatic cleavage of the isolated compound yielded estriol and glucuronic acid.
