Erythrocentaurin

Zhongguo Zhong Yao Za Zhi. 2015 Oct;40(20):4012-7.

Chemical constituents of Swertia patens.[Pubmed: 27062819]

Chemical constituents of Swertia patens. The whole plant of air-dried Swertia patens was extracted with 90% EtOH.
METHODS AND RESULTS:
The water extract was suspended in H₂O and extracted with petroleum ether, EtOAc and n-BuOH, successively. The compounds were isola- ted and purified by column chromatography from the EtOAc fraction, and identified based on spectral analyses (MS, ¹H-NMR, ¹³C- NMR). Eighteen compounds were isolated and elucidated as 3, 4-dihydro-1H,6H,8H-naptho [1,2-c:4,5-c', d'dipyrano-1, 8-dione (1), angelone (2), gentiogenal (3), erythricin (4), Erythrocentaurin (5), gentianine (6), swertiakoside B (7), swertiamarin (8), 2'-O-actylswertiamarin (9), amarogentin (10), 1, 3, 5-trihydroxyxanthone (11), 1, 3-dihydroxy-5-methoxyxanthone (12), 1-hydroxy- 2, 3, 5-trimethoxyxanthone (13), gentiocrucine (14), 3-hydroxyphenylketone (15), n-hexacosyl ester 4-hydroxy-trans-cinnamate (16), n-hexacosyl ester 4-hydroxy-cis-cinnamate (17), and cholest-4-en-3-one (18).
CONCLUSIONS:
Compounds 1-7, 9-18 were obtained from S. patens for the first time.

Nat Prod Res. 2006 Aug;20(10):896-901.

Phytochemical studies and antibacterial activity of Centaurium pulchellum Druce.[Pubmed: 16854716 ]

METHODS AND RESULTS:
Two isocoumarin derivatives, one new, erythricin (1) and a known Erythrocentaurin (2) were isolated from the whole plant of Centaurium pulchellum Druce. The 13C-NMR data of compound 2 are described. The structures of compounds 1, 2 were elucidated on the basis of spectral analysis including 2D-NMR experiments.
CONCLUSIONS:
Antibacterial and brine shrimp lethality assays are also described on the fractions of the plant extract.

J Sep Sci. 2015 Feb;38(4):592-8.

Rapid preparative isolation of erythrocentaurin from Enicostemma littorale by medium-pressure liquid chromatography, its estimation by high-pressure thin-layer chromatography, and its α-amylase inhibitory activity.[Pubmed: 25504557]

Erythrocentaurin is a relatively simple natural product present among the members of Gentianaceae. A preparative method for the isolation of Erythrocentaurin from the ethyl acetate fraction of Enicostemma littorale using medium-pressure liquid chromatography has been reported.
METHODS AND RESULTS:
The method consisted of a simple step gradient from 10 to 20% ethyl acetate in n-hexane. Using a 70 × 460 mm Si60 column, this method is capable of processing 20 g of material in <3 h (purity ≈ 97%). The recovery of Erythrocentaurin was 87.77%. Estimation of Erythrocentaurin in extracts and fractions based on high-pressure thin-layer chromatography was carried out on silica gel 60 F(254) plates with toluene/ethyl acetate/formic acid (80:18:2 v/v/v) as the mobile phase. The densitometric analysis was performed at 230 nm. A well-separated compact band of Erythrocentaurin appeared at R(f )0.54 ± 0.04. The analytical method showed good linearity in the concentration range of 200-1500 ng/band with a correlation coefficient of 0.99417.
CONCLUSIONS:
The limits of detection and quantification were found to be ≈60 and ≈180 ng/band, respectively. Erythrocentaurin exhibited a concentration-dependent α-amylase inhibition (IC(50) 1.67 ± 0.28 mg/mL). The outcome of the study should be considered for pharmacokinetic and biotransformation studies involving E. littorale.