Deoxytrillenoside A
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Study on Chemical Components in the Roots and Rhizome of Trillium tschonoskii(Ⅴ)[Reference:
WebLink]
To investigate the chemical constituents in the roots and rhizome of Trillium tschonoskii Maxim.
METHODS AND RESULTS:
The 85% methanol elution parts from the roots and rhizome of Trillium tschonoskii Maxim were separated and purified by polyamide,silica gel,RP- C18,Sephadex LH- 20 column chromatography and the preparation of high performance liquid,et al.Chemical structures were identified by MS,1D and 2D NMR experiments. Nine compounds were isolated and identified from the 85% methanol elution parts of Trillium tschonoskii Maxim as 26- O- β- D- glucopyranosyl-( 25s)- 5α- furostan-△20( 22)- ene- 3β,26- glycol- 3- O- α- L- rhamnopyranosyl-( 1 →4)- [α- L- rhamnopyranosyl( 1 →2) ]- β- D-glucopyranoside( 1),26- O- β- D- glucopyranosyl-( 25s)- 5α- furostan- △20( 22)- ene- 3β,26- glycol- 3- O- α- L- rhamnopyranosyl-( 1→4)- β- D- glucopyranoside( 2),26- O- β- D- glucopyranosyl-( 25s)- 5α- furostan- △5( 6),20( 22)- diene- 3β,26- glycol- 3- O- α- L- rhamnopyranosyl-( 1 →4)- β- D- glucopyranoside( 3),Deoxytrillenoside A( 4),24- acetyl- Deoxytrillenoside A( 5),24- methoxy- trillenoside A( 6),trillenoside B( 7),24- acetyl- trillenoside B( 8),24- acetyl- deoxytrillenoside B( 9).
CONCLUSIONS:
Compounds 1 ~ 3 for the first time isolated from this genus,compounds5,6,8 and 9 for the first time isolated from this plant.
