Complanatoside A

Biomed Chromatogr. 2016 Jun;30(6):888-93.

Quantification of complanatoside A in rat plasma using LC-MS/MS and its application to a pharmacokinetic study.[Pubmed: 26393341]

Complanatoside A is a flavonol glycoside isolated from Astragalus complanatus, and currently it is used as a quality control index for A. complanatus in the 2010 edition of the Chinese Pharmacopoeia.
METHODS AND RESULTS:
For the first time, a simple and sensitive LC-MS/MS method was developed for the determination of Complanatoside A in rat plasma over the range of 2.3-575 ng/mL. Complanatoside A was extracted from plasma by a protein precipitation procedure, separated by LC and detected by MS/MS in positive electrospray ionization mode. The method was validated for selectivity, carryover, sensitivity, linearity, extraction recovery, matrix effect, accuracy, precision and stability studies. The lower limit of quantification was established at 2.3 ng/mL. Intra- and inter-day precisions (LLOQ, low-QC, med-QC and high-QC) were <7.9%, and accuracies were between 94.0 and 105.1%. Matrix effect was acceptable (97.9-103.0%) and extraction recovery was reproducible (88.5-94.4%). Complanatoside A was stable in the investigated conditions.
CONCLUSIONS:
The method was applied to the pharmacokinetics of Complanatoside A in rats.

Zhongguo Zhong Yao Za Zhi. 2005 Apr;30(8):600-2.

[Determination of complanatoside A in semen Astragali complanati by HPLC].[Pubmed: 16011285]

To establish the determination method for Complanatoside A in seeds of Astragalus complanatus.
METHODS AND RESULTS:
An HPLC method has been developed to separate Complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm. The good linearity of Complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%.
METHODS AND RESULTS:
The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.