Bakkenolide Db
Bakkenolide Db may have cytotoxicity.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chem Pharm Bull (Tokyo). 1999 Mar;47(3):375-82.
The bakkenolides from the root of Petasites formosanus and their cytotoxicity.[Pubmed:
10400491]
METHODS AND RESULTS:
Thirty-two new bakkenolides, Bakkenolide Db (1) bakkenolide Dh(7), bakkenolide Fa(8), bakkenolide Fb(9), bakkenolide I(10) bakkenolide M(14), bakkenolide Na(15), bakkenolide Nb(16), bakkenolide O(17) bakkenolide T(22), bakkenolide Ua (23), bakkenolide Ub(24), bakkenolide V(25) bakkenolide X(27), bakkenolide Ya(28), bakkenolide Yb(29), bakkenolide Za(30), bakkenolide Zb(31) and bakkenolide III(32), from the roots of Petasites formosanus together with thirty known compounds were isolated. The structures were characterized by spectral analysis.
CONCLUSIONS:
The locations, C-1 and/or C-9 of bakkenolide skeleton, of the substituents, such as acetoxy, isobutyroyloxy and isovaleroyloxy groups, can be determined by the chemical shifts of their signals and the H-1 and/H-9 in the 1H-NMR spectra. The cytotoxicity was also discussed.
