6-Hydroxykaempferol 3-beta-rutinoside
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Journal of Chinese Pharmaceutical Sciences, 2014, 23(7).
HPLC fingerprint-oriented preparative separation of major flavonoids from safflower extract by preparative pressurized liquid chromatography[Reference:
WebLink]
The aim of this study was to rapidly isolate the major effective flavanoids from the extract of safflower(Carthamus tinctorius) using ODS medium pressure liquid chromatography(MPLC) and semi-preparative HPLC, guided by a developed fingerprint.
METHODS AND RESULTS:
Twelve compounds were isolated and their structures were elucidated as kaempferol 3-O-β-D-rutinoside(1),
kaempferol 3-O-β-D-glucoside(2), rutin(3), quercetin 3-O-β-D-glucoside(4), 6-hydroxykaempferol 3,6,7-tri-O-β-D-glucoside(5), 6-hydroxykaempferol 3-O-β-D-glucoside(6-Hydroxykaempferol 3-beta-rutinoside, 6), 6-hydroxykaempferol 6,7-di-O-β-D-glucoside(7), 6-hydroxykaempferol 3-O-β-Drutinoside(8), 6-hydroxykaempferol 3,6-di-O-β-D-glucosyl 7-O-β-D-glucuronide(9), isosafflomin C(10), safflomin C(11) and hydroxysafflor yellow A(12) by spectroscopic analysis and comparing with the literature.
CONCLUSIONS:
Our results demonstrated that preparative pressurized liquid chromatography combined with HPLC fingerprint guide is an efficient tool to isolate the target compounds quickly.
