3'-O-Methylorobol
3'-O-Methylorobol exhibits moderate antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, it also exerts potential analgesic properties. 3'-O-Methylorobol can increase osteoblast differentiation.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Phytother Res. 2009 Jan;23(1):129-31.
Stimulatory constituents of Eclipta prostrata on mouse osteoblast differentiation.[Pubmed:
18683850]
METHODS AND RESULTS:
One flavonoid, diosmetin (1), and two isoflavonoids, 3'-hydroxybiochanin A (2) and 3'-O-Methylorobol (3), were isolated from the methanol extract of Eclipta prostrata L. by a bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system.
CONCLUSIONS:
All three compounds significantly increased osteoblast differentiation as assessed by the alkaline phosphatase activity.
J Asian Nat Prod Res. 2010 Feb;12(2):138-43.
Antioxidant flavonoids from Alhagi maurorum.[Pubmed:
20390757]
METHODS AND RESULTS:
A new flavonoid, isorhamnetin-3-O-[-alpha-l-rhamnopyranosyl-(1 --> 3)]-beta-D-glucopyranoside (1), along with two known flavonoids 3'-O-Methylorobol (2) and quercetin 3-O-beta-d-glucopyranoside (3), was isolated from Alhagi maurorum. Their structures were established with the help of mass spectrometry, 1D and 2D NMR spectroscopy, and in comparison with the literature data.
CONCLUSIONS:
Compounds 1 and 2 exhibited moderate antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay.
Records of Natural Products, 2013, 7(3): 169-76.
Analgesic and Antioxidant Activities of Algerian Retama raetam (Forssk.) Webb & Berthel Extracts.[Reference:
WebLink]
METHODS AND RESULTS:
Part of this work deals with the isolation and structure elucidation of the main polar secondary metabolites of the aerial parts of Retama raetam (Forssk.) Webb & Berthel, as well as the evaluation of their potential analgesic properties, while the rest deals with the antioxidant activities of the aqueous extracts of roots, stem, fruits and flowers of the plant. It was found that the isoflavones genistein 1, 6-hydroxygenistein 2, 3'-O-Methylorobol 3, pratensein 4, biochanin A 8, the flavones 6-hydroxyapigenin 7 and luteolin 5, the flavonol kaempferol 6, as well as the phenolic compound p-coumaric acid 9 reduce significantly the pain at a concentration dose of 1 mg/kg. The most active compounds were 3 and 8 (86.19% and 75.23%, respectively).
CONCLUSIONS:
The obtained aqueous extracts of R. raetam were also evaluated for their antioxidant activities using two different photometric methods; the results revealed that all extracts exerted very low free radical scavenging activity compared to the well-known butylated hydroxytoluene (BHT) and lower hydrogen peroxide blocking activity than positive control gallic acid.
