Uvarigrin
Uvarigrin shows cytotoxicity against HCT-8, Bel7402 and A2780 human tumor cell lines at ED50 levels of 0.15, 0.21 and 0.41 microgram/ml, respectively. It induces apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.
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24 months(2-8C).
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Yao Xue Xue Bao. 2006 Mar;41(3):252-6.
Induction of apoptosis of tumor multidrug resistance cell by uvarigrin and its mechanism.[Pubmed:
16758998]
To study the effect of Uvarigrin on mitochondrial dependent pathway during the apoptosis induced by it in MDR KBv200 cells and their parental sensitive KB cells.
METHODS AND RESULTS:
MTT assay was used to detect the cytotoxic effect of Uvarigrin on KBv200 and KB cells. Annexin V FITC staining identified Uvarigrin-induced apoptosis in KBv200 and KB cells. These cells underwent incubation with DCFH-DA, or DiOC6, followed by flowcytometry for the measurement of reactive oxygen species (ROS) and mitochondrial membrane potential (deltapsim), respectively. The Western blotting analysis was performed on Caspase-9 activation. Uvarigrin inhibited the growth of KBv200 cells and KB cells in vitro. Most of the Uvarigrin-induced cells death was found to be due to apoptosis, as determined by Annexin V FITC staining. During the apoptosis, the level of ROS increased while the level of deltapsim decreased in a time-dependent manner. Uvarigrin triggered Caspase-9 activation.
CONCLUSIONS:
Uvarigrin induced apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.
Yao Xue Xue Bao. 1997 Apr;32(4):286-93.
Studies on new cytotoxic annonaceous acetogenins from Uvaria grandiflora and absolute configurations.[Pubmed:
11499032]
Two new cytotoxic annonaceous acetogenins, named Uvarigrin(1) and uvarigrandin A(3), were obtained from the roots of Uvaria grandiflora Roxb(Annonaceae).
METHODS AND RESULTS:
Based on X-ray analysis and Mosher's methodology, the overall absolute configuration of 1 was established as 15S, 17R, 18R, 21R, 22R, 36S. The absolute configuration of 3 was also resolved by Mosher's methodology. The relative configuration of the previously reported uvarigranin (2) was revised.
CONCLUSIONS:
Compound 1 showed cytotoxicity against HCT-8, Bel7402 and A2780 human tumor cell lines at ED50 levels of 0.15, 0.21 and 0.41 microgram.ml-1, respectively.
