Uvarigrin
Uvarigrin shows cytotoxicity against HCT-8, Bel7402 and A2780 human tumor cell lines at ED50 levels of 0.15, 0.21 and 0.41 microgram/ml, respectively. It induces apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Yao Xue Xue Bao. 2006 Mar;41(3):252-6.
Induction of apoptosis of tumor multidrug resistance cell by uvarigrin and its mechanism.[Pubmed:
16758998]
To study the effect of Uvarigrin on mitochondrial dependent pathway during the apoptosis induced by it in MDR KBv200 cells and their parental sensitive KB cells.
METHODS AND RESULTS:
MTT assay was used to detect the cytotoxic effect of Uvarigrin on KBv200 and KB cells. Annexin V FITC staining identified Uvarigrin-induced apoptosis in KBv200 and KB cells. These cells underwent incubation with DCFH-DA, or DiOC6, followed by flowcytometry for the measurement of reactive oxygen species (ROS) and mitochondrial membrane potential (deltapsim), respectively. The Western blotting analysis was performed on Caspase-9 activation. Uvarigrin inhibited the growth of KBv200 cells and KB cells in vitro. Most of the Uvarigrin-induced cells death was found to be due to apoptosis, as determined by Annexin V FITC staining. During the apoptosis, the level of ROS increased while the level of deltapsim decreased in a time-dependent manner. Uvarigrin triggered Caspase-9 activation.
CONCLUSIONS:
Uvarigrin induced apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.
Yao Xue Xue Bao. 1997 Apr;32(4):286-93.
Studies on new cytotoxic annonaceous acetogenins from Uvaria grandiflora and absolute configurations.[Pubmed:
11499032]
Two new cytotoxic annonaceous acetogenins, named Uvarigrin(1) and uvarigrandin A(3), were obtained from the roots of Uvaria grandiflora Roxb(Annonaceae).
METHODS AND RESULTS:
Based on X-ray analysis and Mosher's methodology, the overall absolute configuration of 1 was established as 15S, 17R, 18R, 21R, 22R, 36S. The absolute configuration of 3 was also resolved by Mosher's methodology. The relative configuration of the previously reported uvarigranin (2) was revised.
CONCLUSIONS:
Compound 1 showed cytotoxicity against HCT-8, Bel7402 and A2780 human tumor cell lines at ED50 levels of 0.15, 0.21 and 0.41 microgram.ml-1, respectively.
