Triumbelletin

Natural Product Research & Development, 2014 , 26 (6) :851-5.

Phenolic Constituents from Rhizome of Wikstroemia indica and Their Anti-tumor Activity[Reference: WebLink]

METHODS AND RESULTS:
Seventeen phenolic constituents were isolated from the rhizome of Wikstroemia indica using a combination of various chromatographic methods. Their structures were elucidated and identified as wikstromol( 1),matairesinol( 2),syringaresinol( 3),pinoresinol( 4),isolariciresinol( 5),ciwujiatone( 6),isorhamnetin-3-O-robinobioside( 7),wikstaiwanone A( 8),wikstaiwanone B( 9),kaempferol( 10),rutin( 11),daphnoretin( 12),Triumbelletin( 13),alnusdiol( 14),aloe-emodin-8-O-β-D-glucoside( 15),chlorogenic acid( 16) and p-hydroxybenzoic acid( 17) by physicochemical properties and spectral data including NMR and MS. Compounds 3-9 and 13-16 were isolated from this plant for the first time.
CONCLUSIONS:
The anti-tumor tests showed compounds 1-4,6-9 exhibited different activities against colon cancer cell lines SW480 and SW620,in which compound 6 showed most significant activities against the two cell lines.

J Sep Sci. 2015 Jun;38(12):2093-100.

Ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry coupled with hierarchical cluster analysis to evaluate Wikstroemia indica (L.) C. A. Mey. from different geographical regions.[Pubmed: 25866087]

METHODS AND RESULTS:
A sensitive, rapid and simple ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry method was developed to determine seven constituents (umbelliferone, apigenin, Triumbelletin, daphnoretin, arctigenin, genkwanin and emodin) in Wikstroemia indica (L.) C. A. Mey. The chromatographic analysis was performed on an ACQUITY UPLC® BEH C18 column (2.1 × 50 mm, 1.7 μm) by gradient elution with the mobile phase of 0.05% formic acid aqueous solution (A) and acetonitrile (B).
CONCLUSIONS:
Multiple reaction monitoring mode with positive and negative electrospray ionization interface was carried out to detect the components. This method was validated in terms of specificity, linearity, accuracy, precision and stability.