Tombozine
Tombozine is a natural product from Rauvolfia yunnanensis.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chemistry of Natural Compounds,2000 ,36 (5) :540-540
Alkaloids of Vinca minor[Reference:
WebLink]
from the aerial part of the plant (3).
METHODS AND RESULTS:
We investigated alkaloids of the aerial part of V. minor cultivated in the Tashkent Botanical Garden during flowering. The aerial part (0.105 kg) was collected in June, 1997, wetted with aqueous ammonia (8%), left for 2 h, and extracted seven times with CHCl . Alkaloids were extracted from the condensed CHCl extracts by H SO (10%). The acid extract was 33 2 4 basicified with NaOH (10%) and extracted with CHCl . Then the alkaline solution was treated with ammonium chloride. The 3 phenolic part was extracted with CHCl . 3 The nonphenolic part (CHCl ) yielded 0.53 g; the phenolic part, 0.12 g. The overall yield was 0.65 g or 0.64% of the 3 dry weight. Column chromatography on silica gel of the nonphenolic part afforded vinc amine (3, 4), akuammicine (3, 5), reserpine (3, 6), majdine (3, 6), reserpinine (3, 6), vinerine (3, 6), ervine (3, 6), vineridine (3, 6), Tombozine (3, 6), vincamajine (3 , 6), and vincanine (3, 6). Column chromatography on silica gel of the phenolic part gave vincanidine (3, 6).
CONCLUSIONS:
All iso lated bases were identified by direct comparison of mixed melting points and IR and UV spectra with authentic samples. The last nine alkaloids were isolated for the first time from the examined species.
