Pseudobufarenogin

Pseudobufarenogin
Product Name Pseudobufarenogin
CAS No.: 17008-69-4
Catalog No.: CFN91017
Molecular Formula: C24H32O6
Molecular Weight: 416.51 g/mol
Purity: >=98%
Type of Compound: Steroids
Physical Desc.: Powder
Targets: Sodium Channel | ATPase | Potassium Channel | MEK | ERK | PI3K | Akt | EGFR | Raf
Source: The glandular body of Bufo bufo
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Price: $268/10mg
Pseudobufarenogin(ψ-bufarenogin), a novel anti-tumor compound, suppresses liver cancer growth by inhibiting receptor tyrosine kinase-mediated signaling. ψ-Bufarenogin shows inhibition of human kidney Na(+)/K(+)-ATPase activity.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
  • Nat Commun.2023, 14(1):5075.
  • Front Pharmacol.2020, 11:683.
  • Drug Chem Toxicol.2024,1-12.
  • Planta Medica International2022, 9(01):e108-e115.
  • J of Applied Biological Chem.2020, 63(2):147-152
  • Anticancer Res.2024, 44(3):1033-1044.
  • J Neuroinflammation.2023, 20(1):268.
  • Preprints2022, 2022030063.
  • Indian J Pharm Sci.2024, 86(2):736-741.
  • Biochem Biophys Res Commun.2018, 505(4):1148-1153
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    Toxicon. 2016 Feb;110:27-34.
    Bufadienolides from parotoid gland secretions of Cuban toad Peltophryne fustiger (Bufonidae): Inhibition of human kidney Na(+)/K(+)-ATPase activity.[Pubmed: 26615828]
    Parotoid gland secretions of toad species are a vast reservoir of bioactive molecules with a wide range of biological properties.
    METHODS AND RESULTS:
    Herein, for the first time, it is described the isolation by preparative reversed-phase HPLC and the structure elucidation by NMR spectroscopy and/or mass spectrometry of nine major bufadienolides from parotoid gland secretions of the Cuban endemic toad Peltophryne fustiger: ψ-bufarenogin(Pseudobufarenogin), gamabufotalin, bufarenogin, arenobufagin, 3-(N-suberoylargininyl) marinobufagin, bufotalinin, telocinobufagin, marinobufagin and bufalin. In addition, the secretion was analyzed by UPLC-MS/MS which also allowed the identification of azelayl arginine. The effect of arenobufagin, bufalin and ψ-bufarenogin(Pseudobufarenogin) on Na(+)/K(+)-ATPase activity in a human kidney preparation was evaluated. These bufadienolides fully inhibited the Na(+)/K(+)-ATPase in a concentration-dependent manner, although arenobufagin (IC50 = 28.3 nM) and bufalin (IC50 = 28.7 nM) were 100 times more potent than ψ-bufarenogin (Pseudobufarenogin,IC50 = 3020 nM).
    CONCLUSIONS:
    These results provided evidence about the importance of the hydroxylation at position C-14 in the bufadienolide skeleton for the inhibitory activity on the Na(+)/K(+)-ATPase.
    Oncotarget. 2015 May 10;6(13):11627-39.
    ψ-Bufarenogin, a novel anti-tumor compound, suppresses liver cancer growth by inhibiting receptor tyrosine kinase-mediated signaling.[Pubmed: 25890498 ]
    Resistance of hepatocellular carcinoma (HCC) to existing chemotherapeutic agents largely contributes to the poor prognosis of patients, and discovery of novel anti-HCC drug is in an urgent need.
    METHODS AND RESULTS:
    Herein we report ψ-Bufarenogin(Pseudobufarenogin), a novel active compound that we isolated from the extract of toad skin, exhibited potent therapeutic effect in xenografted human hepatoma without notable side effects. In vitro, ψ-Bufarenogin suppressed HCC cells proliferation through impeding cell cycle progression, and it facilitated cell apoptosis by downregulating Mcl-1 expression. Moreover, ψ-Bufarenogin decreased the number of hepatoma stem cells through Sox2 depression and exhibited synergistic effect with conventional chemotherapeutics. Mechanistic study revealed that ψ-Bufarenogin impaired the activation of MEK/ERK pathway, which is essential in the proliferation of hepatoma cells. ψ-Bufarenogin notably suppressed PI3-K/Akt cascade, which was required in ψ-Bufarenogin-mediated reduction of Mcl-1 and Sox2. ψ-Bufarenogin inhibited the auto-phosphorylation and activation of epithelial growth factor receptor (EGFR) and hepatocyte growth factor receptor (c-Met), thereafter suppressed their primary downstream cascades Raf/MEK/ERK and PI3-K/Akt signaling.
    CONCLUSIONS:
    Taken together, ψ-Bufarenogin suppressed HCC growth via inhibiting, at least partially, receptor tyrosine kinases-regulated signaling, suggesting that ψ-Bufarenogin could be a novel lead compound for anti-HCC drug.
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