Parisyunnanoside B
Reference standards.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Planta Med. 2009 Mar;75(4):356-63.
Steroidal saponins from the rhizome of Paris polyphylla and their cytotoxic activities.[Pubmed:
19085682 ]
Two new furostanol saponins and one new spirostanol saponin were isolated from the rhizome of Paris polyphylla Smith var. yunnanensis, together with 18 known steroidal saponins.
METHODS AND RESULTS:
The structures of the new steroidal saponins were elucidated as 26-O-beta-D-glucopyranosyl-(25R)-5-ene-furost-3 beta, 17 alpha, 22 alpha, 26-tetrol-3-O-alpha-L-arabinofuranosyl-(1-->4)-[alpha-L-rhamnopyranosyl-(1-->2)]-beta-D-glucopyranoside (2, parisyunnanoside A), 26-O-beta-D-glucopyranosyl-(25R)-5, 20 (22)-diene-furost-3 beta, 26-diol-3-O-alpha-L-arabinofuranosyl-(1-->4)-[alpha-L-rhamnopyranosyl-(1-->2)]-beta-D-glucopyranoside (7, Parisyunnanoside B), and (25R)-spirost-5-ene-3 beta, 12 alpha-diol-3-O-alpha-L-rhamnopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->4)-[alpha-L-rhamnopyranosyl-(1-->2)]-beta-D-glucopyranoside (13, parisyunnanoside C) by MS and 1 D and 2 D NMR analysis. The isolated compounds were evaluated for their cytotoxicity against HL-60 human promyelocytic leukemia cells.
CONCLUSIONS:
Our results showed that the spirostanol framework of the aglycone and the terminal alpha-L-rhamnopyranosyl with 1-->2 linkage to the sugar chain of saponins at C-3 are essential for their high cytotoxicity, whereas the hydroxy group substitution at C-12 or C-17 of the aglycone causes a reduction in their activity.
《Journal of Jinan University(Natural Science & Medicine Edition)》 2014-01
Chemical constituents of Paris polyphylla var. yunnanensis[Reference:
WebLink]
METHODS AND RESULTS:
Thirteen compounds were obtained from the 30% ethanol eluting fraction of macroporous resin and their structures were elucidated by their spectral data and physicochemical properties,which were identified as calonysterone( 1),glycoside St-J( 2),methyl ester of glycoside St-J( 3),cussonoside B( 4),methyl 3, 4-dihydroxybenzoate( 5),α-ecdysone( 6),20-hydroxyecdysone( 7),pinnatasterone( 8),polyphyllin H( 9),polyphyllin Ⅶ( 10),parisaponin I( 11),Parisyunnanoside B( 12),polyphyllin Ⅱ( 13).
CONCLUSIONS:
Among them,compounds 1- 5 were obtained from the genus Paris for the first time,and 1- 6 were isolated from this plant for the first time.
