Ophiopogonin C
Ophiopogonin C' pharmacological activities may be related to the substances in nucleus mainly.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Pharmaceutical Biotechnology, 2013-04
Study on the distribution in cells by Ophiopogonin C.[Reference:
WebLink]
To investigate the effect and its feature of location and accumulation of Ophiopogonin C(DT-13) in HUVEC/HeLa cells moreover, a new method for the study of efficacy and mechanism of saponins of Traditional Chinese medicine(TCM) is provided.
METHODS AND RESULTS:
We obtaind and purified enough DT-13 monoclonal antibodies with the pre-obtained DT-13 hybridoma cell line 1B12, its activities with icELISA are also detectad. The effect of proliferation on HUVEC/HeLa cells after DT-13 with different series of concentrations by MTT assay is investigated. The effect and its feature of location and accumulation of DT-13 in HUVEC/HeLa cells by immunflouresence(FITC & DAPI staining) method and studied. The results showed that under the no-cytotoxicitiy concentration of DT-13, it can be carried thraugh in to cells' cytoplasm quickly and then got into nucleus gradually and accumulated for a long time. When in HUVEC cells, DT-13 (5.0 μmol/L) is in cytoplasm 30 min before, 12 h later it is basically all into the nucleus accumulating for at least 24 h. While in HeLa cells, DT-13 (2.5 μmol/L) is faster to get into nucleus than in HUVEC cells, slao accumulate for at least 24 h.
CONCLUSIONS:
The resluts suggest that DT-13' pharmacological activities may be related to the substances in nucleus mainly, and DT-13' main mechanism remains to be further studied. In summary, DT-13' localization and dynamic process in cells can be detected by this method with the advantage of direct-viewing, reliability and convenience. Also, this method can be referred when studying on other saponins of TCM or their analogues.
