Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Chinese Traditional & Herbal Drugs, 2012, 43(1):38-42.
Chemical constituents from Dysoxylum lenticellatum.[Reference:
WebLink]
To study the chemical constituents from the stems of Dysoxylum lenticellatum and their cytotoxic activities.
METHODS AND RESULTS:
Silica gel,RP-18,and Sephadex LH-20 column chromatography techniques were used for separation and purification of the compounds and extensive spectral analysis spectra were employed for structural elucidation.The cytotoxic activity was evaluated by MTT method. Twelve compounds were isolated from D.lenticellatum and their structures were identified as β-sitosterol(1),stigmasterol(2),3-oxo-24,25,26,27-tetranortirucall-7-ene-23(21)-lactone(3),3α-hydroxy-24,25,26,27-tetranortirucall-7-ene-23(21)-lactone(4),Laxiracemosin H(5),laxiracemosin B(6),24,25-epoxy-tirucall-7-ene-3,23-dione(7),butyrospermol(8),oleanolic acid(9),taraxerone(10),taraxerol(11),and 8-hydroxy-6-methoxy-3-pentylisocoumarin(12).
CONCLUSIONS:
All of these compounds are isolated from D.lenticellatum for the first time and the compounds 8,10—12 are reported from the plants in Dysoxylum Bl.for the first time.What’s more,compounds 5 and 6 are tirucallane-type alkaloids and compound 3 shows significant inhibitory activities against human cancer cell lines in vitro.
