Lanatoside B
Lanatoside B is a natural product from Polygonum lapathifolium.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Journal of Liquid Chromatography, 1994,17(20): 4451-61.
High-Performance Liquid Chromatographic Determination of Lanatosides in Digitalis Lutea and Digitalis Ambigua Leaves[Reference:
WebLink]
METHODS AND RESULTS:
An quantitative method for the determination of lanatoside A and Lanatoside B in Digitalis lutea and Digitalis ambigua leaves by high-performance liquid chromatography (HPLC) is described. The extract of dry leaf powder with chloroform:ethanol (1:2, v/v) was submitted to Sep-Pak cartridges prior to HPLC analysis. HPLC was performed on an ODS column using methanol:water (2:1, v/v) for Digitalis lutea and a phenylsilyl bonded silica column with acetonitrile:water (5:8, v/v) for Digitalis ambigua. The effluent was monitored by ultraviolet (UV) absorption at 220 nm. The quantitation was carried out by the internal standard method.
CONCLUSIONS:
The present method is sufficiently sensitive and reproducible to assay lanatosides in Digitalis leaves.
