Hosenkoside A
Hosenkoside A is a nartural product from Impatiens balsamina L.
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Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to
24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Chinese Journal of Pharmaceutical Analysis, 2009(5): 717-20.
RP-HPLC determination of two hosenkosides in seeds of Impatiens balsamina L.[Reference:
WebLink]
To determine two hosenkosides(hosenkoside K and Hosenkoside A)in the seeds of Impatiens balsamina.
METHODS AND RESULTS:
:Using RP-HPLC with ELSD method.Separation was carried out on Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm).A linear solvent gradient was used,with a mobile phase of methanol-water(0-22 min,50∶50;22-50 min,54∶46).The flow rate was 0.8 mL·min-1 at 25 ℃.The gas(air)flow rate was 2.5 L·min-1 and the temperature of drift tube for measurement was 89 ℃.The linearity was in the range of 5-20 μg for hosenkoside K and A,and the correlation coefficients(n=6)were 0.9995 and 0.9992 respectively;The average recoveries(n=6)for hosenkoside K and A were 98.0%(RSD=1.3%)and 98.0%(RSD=1.3%)respectively.
CONCLUSIONS:
This method is accurate,reliable and reproducible for determination of hosenkoside K and A in the seeds of Impatiens balsamina.
