Dehydrocavidine

Org Biomol Chem. 2009 Jul 7;7(13):2699-703.

Selective binding and highly sensitive fluorescent sensor of palmatine and dehydrocorydaline alkaloids by cucurbit[7]uril.[Pubmed: 19532985]

METHODS AND RESULTS:
The complexation behavior of palmatine (P) and Dehydrocorydaline (DHC) alkaloid guest molecules by cucurbit[7]uril (CB7) host have been investigated by means of fluorescence spectra in aqueous phosphate buffer solution (pH 7.2). It is found that each alkaloid exhibits dramatic fluorescence enhancement upon complexation with CB7, and the intensity of the emittance is strong enough to be readily distinguished by the naked eye. Although the two guests possess similar structure, the complex stability constant of P with CB7 is 5.4 times larger than that of DHC.
CONCLUSIONS:
1H NMR studies show that the binding modes differ much, i.e., deep encapsulation for P-CB7 and shallow encapsulation for Dehydrocorydaline-CB7. Furthermore, the solvent effects and salt effects during the course of complexation have also been investigated, showing they significantly influence the binding ability and selectivity of CB7 with the alkaloid guests. Particularly, addition of a small amount (4 vol%) of ethanol increases the P/Dehydrocorydaline selectivity to 17.2.

Molecules. 2012 Jan 18;17(1):951-70.

Effect of wine and vinegar processing of Rhizoma Corydalis on the tissue distribution of tetrahydropalmatine, protopine and dehydrocorydaline in rats.[Pubmed: 22258341]

Abstract Vinegar and wine processing of medicinal plants are two traditional pharmaceutical techniques which have been used for thousands of years in China. Tetrahydropalmatine (THP), Dehydrocorydaline (DHC) and protopine are three major bioactive molecules in Rhizoma Corydalis.
METHODS AND RESULTS:
In this study, a simple and reliable HPLC method was developed for simultaneous analysis of THP, Dehydrocorydaline and protopine in rat tissues after gastric gavage administration of Rhizoma Corydalis. The validated HPLC method was successfully applied to investigate the effect of wine and vinegar processing on the compounds' distribution in rat tissues. Our results showed that processing mainly affect the T(max) and mean residence time (MRT) of the molecules without changing their C(max) and AUC(0-24)( )(h) Vinegar processing significantly increased the T(max) of Dehydrocorydaline in heart, kidney, cerebrum, cerebrellum, brain stem and striatum and prolonged the T(max) of protopine in brain. No significant changes were observed on the T(max) of THP in rat tissues after vinegar processing. Wine processing reduced the T(max) of protopine and Dehydrocorydaline in liver and spleen and T(max) of protopine in lung, but increased the T(max) of THP in all the rat tissues examined.
CONCLUSIONS:
To our knowledge, this is the first report on the effects of processing on the tissue distribution of the bioactive molecules from Rhizoma Corydalis.

Int Immunopharmacol. 2011 Sep;11(9):1362-7.

Dehydrocorydaline inhibits elevated mitochondrial membrane potential in lipopolysaccharide-stimulated macrophages.[Pubmed: 21575743]

Activated macrophages play a critical role in the pathogenesis of numerous diseases by producing pro-inflammatory cytokines such as interleukin (IL)-1β and IL-6. While the mechanisms of bacterial component recognition and signal transduction have been well investigated, viability regulation in activated macrophages remains unclear.
METHODS AND RESULTS:
We screened herbal ingredients to find an agent that reduces the viability of lipopolysaccharide (LPS)-stimulated macrophages and observed that Dehydrocorydaline, a component of Corydalis yanhusuo, reduced the viability of macrophage-derived RAW264.7 cells and primary macrophages in the presence of LPS. Dehydrocorydaline inhibited the elevation of mitochondrial membrane potential and induced ATP depletion in LPS-stimulated macrophages but neither affected basal mitochondrial membrane potential nor ATP content in non-stimulated macrophages. Dehydrocorydaline also prevented increased concentrations of IL-1β and IL-6 in culture media of LPS-stimulated macrophages.
CONCLUSIONS:
Mode of Dehydrocorydaline action indicates that elevated mitochondrial membrane potential may be a novel target to specifically reduce viability and suppress cytokine production in LPS-stimulated macrophages.

Am J Chin Med. 2012;40(1):177-85.

Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells.[Pubmed: 22298457]

Dehydrocorydaline is an alkaloid isolated from traditional Chinese herb Corydalis yanhusuo W.T. Wang. We discovered that it possessed anti-tumor potential during screening of anti-tumor natural products from Chinese medicine.
METHODS AND RESULTS:
In this study, its anti-tumor potential was investigated with breast cancer line cells MCF-7 in vitro. The anti-proliferative effect of Dehydrocorydaline was determined by MTT assay and the mitochondrial membrane potential (Δ Ψ m) was monitored by JC-1 staining. DNA fragments were visualized by Hoechst 33342 staining and DNA ladder assay. Apoptotic related protein expressions were measured by Western blotting. Dehydrocorydaline significantly inhibited MCF-7 cell proliferation in a dose- dependent manner, which could be reversed by a caspase-8 inhibitor, Z-IETD-FMK. Dehydrocorydaline increased DNA fragments without affecting ΔΨm. Western blotting assay showed that Dehydrocorydaline dose-dependently increased Bax protein expression and decreased Bcl-2 protein expression. Furthermore, Dehydrocorydaline induced activation of caspase-7,-8 and the cleavage of PARP without affecting caspase-9.
CONCLUSIONS:
These results showed that Dehydrocorydaline inhibits MCF-7 cell proliferation by inducing apoptosis mediated by regulating Bax/Bcl-2, activating caspases as well as cleaving PARP.