Cryptotanshinone

Cryptotanshinone
Product Name Cryptotanshinone
CAS No.: 35825-57-1
Catalog No.: CFN98478
Molecular Formula: C19H20O3
Molecular Weight: 296.4 g/mol
Purity: >=98%
Type of Compound: Diterpenoids
Physical Desc.: Red powder
Targets: NF-kB | TNF-α | ROS | Caspase | p38MAPK | Akt | P450 (e.g. CYP17) | JNK | MMP(e.g.TIMP) | AP-1 | Androgen Receptor | ERK | PI3K | Bcl-2/Bax | COX
Source: The roots of Salvia miltiorrhiza Bge.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Price: $30/20mg
Cryptotanshinone is a potent STAT3 inhibitor with IC50 of 4.6 μM, and inhibits STAT3 Tyr705 phosphorylation in DU145 prostate cancer cells. It is also an AR inhibitor to suppress androgen/AR-mediated cell growth and PSA expression by blocking AR dimerization and the AR-coregulator complex formation. Cryptotanshinone has anti-atherosclerosis, neuroprotective, anti-cancer,and anti-neointimal formation activities. Cryptotanshinone reverses DEX-induced androgen excess and ovarian IR in mice through activation of insulin signaling and the regulation of glucose transporters and hormone-synthesizing enzymes, it has an inhibitory effect on MMP-9 production and migration of human aortic smooth muscle cells treated with TNF-alpha in a dose-dependent manner.
Inquire / Order: manager@chemfaces.com
Technical Inquiries: service@chemfaces.com
Tel: +86-27-84237783
Fax: +86-27-84254680

Address:
1 Building, No. 83, CheCheng Rd., Wuhan Economic and Technological Development Zone, Wuhan, Hubei 430056, PRC
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
  • Phytochem Anal.2016 Sep;
  • Tea Res. Ins. Of ChinaJuly 13, 2017;
  • Nat Plants. 2016 Dec 22
  • Chem Pharm Bull (Tokyo).2017;65(9)
  • Evid Based Complement Alternat Med. 2017
  • Sci Rep. 2017 Dec 11;
  • J Med Food. 2016 Dec;19(12)
  • Analytical sci. & Tech2016
  • J. of The Korean Society of Food CultureApr.2017;
  • Sci Rep. 2017 Apr 11;
  • Dehydromiltirone

    Catalog No: CFN98532
    CAS No: 116064-77-8
    Price: $520/5mg
    Miltirone

    Catalog No: CFN98531
    CAS No: 27210-57-7
    Price: $288/5mg
    1,2-Didehydrocryptotanshinone

    Catalog No: CFN92144
    CAS No: 891854-92-5
    Price: Inquiry(manager@chemfaces.com)
    Cryptotanshinone

    Catalog No: CFN98478
    CAS No: 35825-57-1
    Price: $30/20mg
    Salvianan

    Catalog No: CFN92637
    CAS No: 862832-46-0
    Price: Inquiry(manager@chemfaces.com)
    Trijuganone C

    Catalog No: CFN92780
    CAS No: 135247-94-8
    Price: Inquiry(manager@chemfaces.com)
    Danshenol B

    Catalog No: CFN90474
    CAS No: 189308-09-6
    Price: Inquiry(manager@chemfaces.com)
    Salviolone

    Catalog No: CFN92163
    CAS No: 119400-86-1
    Price: $595/5mg
    Apoptosis. 2011 Jul;16(7):696-707.
    Cryptotanshinone enhances TNF-α-induced apoptosis in chronic myeloid leukemia KBM-5 cells.[Pubmed: 21519916]
    Cryptotanshinone is a biologically active compound from the root of Salvia miltiorrhiza. In the present study, we investigated the molecular mechanisms by which Cryptotanshinone is in synergy with tumor necrosis factor-alpha (TNF-α) for the induction of apoptosis in human chronic myeloid leukemia (CML) KBM-5 cells.
    METHODS AND RESULTS:
    The co-treatment of Cryptotanshinone with TNF-α reduced the viability of the cells [combination index (CI) < 1]. Concomitantly, the co-treatment of Cryptotanshinone and TNF-α elicited apoptosis, manifested by enhanced the number of terminal deoxynucleotide transferase-mediated dUTP-nick-end labeling (TUNEL)-positive cells, the sub-G1 cell populations, and the activation of caspase-8 and -3, in comparison with the treatment with either drug alone. The treatment with Cryptotanshinone further suppressed TNF-α-mediated expression of c-FLIP(L), Bcl-x(L), but the increased level of tBid (a caspase-8 substrate). Furthermore, Cryptotanshinone activated p38 but not NF-κB in TNF-α-treated KBM-5 cells. The addition of a specific p38 MAPK inhibitor SB203580 significantly attenuated Cryptotanshinone/TNF-α-induced apoptosis. The combination treatment of Cryptotanshinone and TNF-α also stimulated the reactive oxygen species (ROS) generation. N-acetyl-L-cysteine (NAC, a ROS scavenger) was not only able to block Cryptotanshinone/TNF-α-induced ROS production but also the activation of caspase-8 and p38 MAPK.
    CONCLUSIONS:
    Overall, our findings suggest that Cryptotanshinone can sensitize TNF-α-induced apoptosis in human myeloid leukemia KBM-5 cells, which appears through ROS-dependent activation of caspase-8 and p38.
    Exp Brain Res. 2009 Feb;193(1):109-18.
    Cryptotanshinone protects primary rat cortical neurons from glutamate-induced neurotoxicity via the activation of the phosphatidylinositol 3-kinase/Akt signaling pathway.[Pubmed: 18936923]
    Excitotoxicity contributes to neuronal death and is involved in the pathogenesis of neurodegenerative disorders such as Alzheimer's disease (AD). In the present study, Cryptotanshinone, an active ingredient from a Chinese plant, Salvia miltiorrhiza, was investigated to assess its neuroprotective effects against glutamate-induced toxicity in primary culture of rat cortical neurons.
    METHODS AND RESULTS:
    Cryptotanshinone reversed glutamate-induced neuronal toxicity, which was characterized by decreased cell viability, increased lactate dehydrogenase release, neuronal DNA condensation, and the alteration of the expression of Bcl-2 family proteins. The neuroprotective effects of Cryptotanshinone could be blocked by LY294002 and wortmannin, two inhibitors of PI3K. The importance of the PI3K pathway was further confirmed by the activation of Akt and anti-apoptotic Bcl-2 by Cryptotanshinone in a PI3K-dependent manner.
    CONCLUSIONS:
    These results suggest that Cryptotanshinone protects primary cortical neurons from glutamate-induced neurotoxicity through the activation of PI3K/Akt pathway. Such neuroprotective effects may be of interest in AD and other neurodegenerative diseases.
    Fertil Steril. 2014 Aug;102(2):589-596.e4.
    Cryptotanshinone reverses ovarian insulin resistance in mice through activation of insulin signaling and the regulation of glucose transporters and hormone synthesizing enzymes.[Pubmed: 24973798]
    To investigate the effects of Cryptotanshinone (CRY), an active component of Chinese medicine, on ovarian androgen production, insulin resistance (IR), and glucose metabolism in mice.
    METHODS AND RESULTS:
    Animal model and in vitro tissue model. University-affiliated laboratory. Ovarian IR was induced by dexamethasone (DEX) in vivo. Animals were randomized to receive CRY treatment for 3 days or not. Ovulation rates, serum steroid levels, and glucose uptake in ovaries were quantified, and proteins in the phosphatidylinositol 3-hydroxy kinase pathway were measured. In vitro ovarian IR was also induced by DEX for 3 days. Ovarian steroid hormone secretion and glucose uptake were measured, and the hormone-synthesizing enzymes were determined by semiquantitative reverse transcription-polymerase chain reaction. Ovarian glucose uptake, in vivo ovulation rate, serum and culture medium steroid level, and molecular expression of phosphatidylinositol 3-hydroxy kinase and steroidogenic enzymes. Dexamethasone significantly increased ovulation rates in vivo and increased T and E2 production and decreased ovarian glucose uptake in vivo and in vitro. Cryptotanshinone significantly reduced ovulation rates in vivo and decreased T and estrogen production in vitro. Cryptotanshinone attenuated the inhibition of DEX on AKT2 and suppressed the up-regulation of CYP11 and CYP17 expression by DEX.
    CONCLUSIONS:
    Cryptotanshinone reversed DEX-induced androgen excess and ovarian IR in mice through activation of insulin signaling and the regulation of glucose transporters and hormone-synthesizing enzymes. This suggests a potential role for CRY in treating the ovulatory dysfunction associated with PCOS.
    Apoptosis. 2012 Mar;17(3):248-57.
    Cryptotanshinone induces ER stress-mediated apoptosis in HepG2 and MCF7 cells.[Pubmed: 22113823 ]
    The endoplasmic reticulum (ER) is a central organelle in eukaryotic cells that functions in protein synthesis and maturation, and also functions as a calcium storage organelle. Perturbation of ER functions leads to ER stress, which has been previously associated with a broad variety of diseases. ER stress is generally regarded as compensatory, but prolonged ER stress can activate apoptotic pathways in damaged cells. For this reason, pharmacological interventions that effectively enhance tumor death through ER stress have been the subject of a great deal of attention for anti-cancer therapy. Cryptotanshinone, the major active constituent isolated from the root of Salvia miltiorrhiza Bunge, has been recently evaluated for its anti-cancer activity, but the molecular mechanisms underlying these activities remain poorly understood. In particular, it remains completely unknown as to whether or not Cryptotanshinone can induce ER stress.
    METHODS AND RESULTS:
    Herein, we identify Cryptotanshinone as a potent stimulator of ER stress, leading to apoptosis in many cancer cell lines, including HepG2 hepatoma and MCF7 breast carcinoma, and also demonstrate that mitogen-activated protein kinases function as mediators in this process.
    CONCLUSIONS:
    Reactive oxygen species generated by Cryptotanshinone have been shown to play a critical role in ER stress-induced apoptosis. Cryptotanshinone also evidenced sensitizing effects to a broad range of anti-cancer agents including Fas/Apo-1, TNF-α, cisplatin, etoposide or 5-FU through inducing ER stress, highlighting the therapeutic potential in the treatment of human hepatoma and breast cancer.
    Biochem Pharmacol. 2006 Dec 15;72(12):1680-9.
    Cryptotanshinone from Salvia miltiorrhiza BUNGE has an inhibitory effect on TNF-alpha-induced matrix metalloproteinase-9 production and HASMC migration via down-regulated NF-kappaB and AP-1.[Pubmed: 16999937 ]
    Matrix metalloproteinases (MMP-9 and MMP-2) production and smooth muscle cell (SMC) migration may play key roles in the phathogenesis of neointima formation and atherosclerosis. Especially inducible MMP-9 expression was directly involved in the cancer cell invasion and SMC migration through vascular wall.
    METHODS AND RESULTS:
    In this study, we reveal that Cryptotanshinone (CT) purified from Salvia miltiorrhiza BUNGE had an inhibitory effect on MMP-9 production and migration of human aortic smooth muscle cells treated with TNF-alpha in a dose-dependent manner. The down regulation of transcription of MMP-9 mRNA was evidenced by RT-PCR and MMP-9 promoter assay using luciferase reporter gene. Eletrophoretic mobility shift assay showed NF-kappaB and AP-1 nuclear translocations were suppressed. In addition, Western blot analysis indicated that extracellular signal regulated kinase 1 and 2, p38 and JNK MAP kinase signaling pathways were inhibited.
    CONCLUSIONS:
    From the results, it is suggested that CT has anti-atherosclerosis and anti-neointimal formation activity.
    Cancer Lett. 2012 Mar;316(1):11-22.
    Cryptotanshinone suppresses androgen receptor-mediated growth in androgen dependent and castration resistant prostate cancer cells.[Pubmed: 22154085]
    Androgen receptor (AR) is the major therapeutic target for the treatment of prostate cancer (PCa). Anti-androgens to reduce or prevent androgens binding to AR are widely used to suppress AR-mediated PCa growth; however, the androgen depletion therapy is only effective for a short period of time.
    METHODS AND RESULTS:
    Here we found a natural product/Chinese herbal medicine Cryptotanshinone (CTS), with a structure similar to dihydrotestosterone (DHT), can effectively inhibit the DHT-induced AR transactivation and prostate cancer cell growth. Our results indicated that 0.5 μM CTS effectively suppresses the growth of AR-positive PCa cells, but has little effect on AR negative PC-3 cells and non-malignant prostate epithelial cells. Furthermore, our data indicated that CTS could modulate AR transactivation and suppress the DHT-mediated AR target genes (PSA, TMPRSS2, and TMEPA1) expression in both androgen responsive PCa LNCaP cells and castration resistant CWR22rv1 cells. Importantly, CTS selectively inhibits AR without repressing the activities of other nuclear receptors, including ERα, GR, and PR.
    CONCLUSIONS:
    The mechanistic studies indicate that CTS functions as an AR inhibitor to suppress androgen/AR-mediated cell growth and PSA expression by blocking AR dimerization and the AR-coregulator complex formation. Furthermore, we showed that CTS effectively inhibits CWR22Rv1 cell growth and expressions of AR target genes in the xenograft animal model. The previously un-described mechanisms of CTS may explain how CTS inhibits the growth of PCa cells and help us to establish new therapeutic concepts for the treatment of PCa.
    Eur J Pharmacol. 2006 Nov 7;549(1-3):166-72.
    Cryptotanshinone inhibits cyclooxygenase-2 enzyme activity but not its expression.[Pubmed: 16989810 ]
    Cyclooxygenase-2 (COX-2) is a key enzyme that catalyzes the biosynthesis of prostaglandins from arachidonic acid and plays a critical role in some pathologies including inflammation, neurodegenerative diseases and cancer. Cryptotanshinone is a major constituent of tanshinones, which are extracted from the medicinal herb Salvia miltiorrhiza Bunge, and has well-documented antioxidative and anti-inflammatory effects.
    METHODS AND RESULTS:
    This study confirmed the remarkable anti-inflammatory effect of Cryptotanshinone in the carrageenan-induced rat paw edema model. Since the action of Cryptotanshinone on COX-2 has not been previously described, in the present study, we further examined the effect of Cryptotanshinone on cyclooxygenase activity in the exogenous arachidonic acid-stimulated insect sf-9 cells, which highly express human COX-2 or human COX-1, and on cyclooxygenases expression in human U937 promonocytes stimulated by lipopolysaccharide (LPS) plus phorbolmyristate acetate (PMA). Cryptotanshinone reduced prostaglandin E2 synthesis and reactive oxygen species generation catalyzed by COX-2, without influencing COX-1 activity in cloned sf-9 cells. In PMA plus LPS-stimulated U937 cells, Cryptotanshinone had negligible effects on the expression of COX-1 and COX-2, at either a mRNA or protein level.
    METHODS AND RESULTS:
    These results demonstrate that the anti-inflammatory effect of Cryptotanshinone is directed against enzymatic activity of COX-2, not against the transcription or translation of the enzyme.
    Agnuside

    Catalog No: CFN99203
    CAS No: 11027-63-7
    Price: $158/10mg
    4,5-Dimethoxycanthin-6-one

    Catalog No: CFN99847
    CAS No: 18110-87-7
    Price: $333/5mg
    3-Hydroxybakuchiol

    Catalog No: CFN92703
    CAS No: 178765-54-3
    Price: $368/10mg
    Bruceine D

    Catalog No: CFN90771
    CAS No: 21499-66-1
    Price: $258/10mg
    1-Methoxyphaseollidin

    Catalog No: CFN96878
    CAS No: 65428-13-9
    Price: $413/5mg
    Angelol M

    Catalog No: CFN95060
    CAS No: 1092952-64-1
    Price: $288/5mg
    Lappaol C

    Catalog No: CFN95068
    CAS No: 64855-00-1
    Price: $333/10mg
    Suspenoidside B

    Catalog No: CFN95071
    CAS No: 2161432-08-0
    Price: $413/5mg
    (1S,2S)-threo-Honokitriol

    Catalog No: CFN95076
    CAS No: 1099687-80-5
    Price: $413/5mg
    Isocarlinoside

    Catalog No: CFN95092
    CAS No: 83151-90-0
    Price: $398/5mg