Asiaticoside B

J Nat Prod. 2006 Oct;69(10):1500-2.

Cytotoxic cycloartane triterpene saponins from Actaea asiatica.[Pubmed: 17067171 ]

METHODS AND RESULTS:
Three new 9,19-cycloartane triterpene glycosides, asiaticoside A (1), Asiaticoside B (2), and 25-O-ethylcimigenol-3-O-beta-D-xylopyranoside (3), together with cimiacemoside I (4), 25-O-acetylcimigenol-3-beta-O-D-xyloside (5), and 25-anhydrocimigenol-beta-O-D-xyloside (6) were isolated from the roots/rhizomes extract of Actaea asiatica, and their structures were established by spectroscopic methods (IR, HRESIMS, and NMR).
CONCLUSIONS:
Compounds 1-3, 5, and 6 had notable cytotoxicity against HepG2 and MCF-7 cancer cell lines.

Pharmazie. 2003 Jun;58(6):381-4.

An improved HPLC method for quantitative determination of six triterpenes in Centella asiatica extracts and commercial products.[Pubmed: 12856998]

METHODS AND RESULTS:
An improved HPLC qualitative and quantitative method of six triterpenes (asiaticoside, madecassoside, asiatic acid, madecassic acid, terminolic acid, and Asiaticoside B) in Centella asiatica (raw plant material and preparations) is described in this paper. After 50 minutes the six active triterpenes were separated and detected in the methanolic extract at a limit of 0.01 microg/ml.
CONCLUSIONS:
The method uses a Phenomenex Aqua 5mu C18 (200 A) column as the stationary phase, a gradient mobile phase of water (0.1% TFA), acetonitrile (0.1% TFA), and methyl tert-butyl ether (0.1% TFA), and UV detection at 206 nm. The correlation coefficients for the calibration curves and the recovery rates ranged from 0.995 to 0.999 and from 98.39% to 100.02%, respectively. The qualitative and quantitative results are discussed.